We narrowed to 5,252 results for: pcDNA3.1+

1. pcDNA3.1-GGamma8-GFP2

   Plasmid

   #140990

   Purpose

   Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma8-GFP2 (GNG8 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. opto-RhoGEF-mCherry_pcDNA3.1

   Plasmid

   #164473

   Purpose

   Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

   Insert

   opto-RhoGEF
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_iSH2-pMagFast2(3x)-iRFP

   Plasmid

   #67298

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   iSH2-pMagFast2(3x)-iRFP (PIK3R1 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MS2-Tet1-CD

   Plasmid

   #83341

   Purpose

   To introduce MS2 coat protein fused Tet1-CD

   Depositor

   Ronggui Hu

   Article

   Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

   Insert

   MS2-Tet1-CD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MBII85-5'SS mut

   Plasmid

   #67631

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutation in 5'ss

   Promoter

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-HsPcdh21-mNeonGreen

   Plasmid

   #210819

   Purpose

   Mammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Protocadherin 21 (CDHR1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-IDH2-R172K

   Plasmid

   #87927

   Purpose

   Expresses IDH2 (R172K mutant) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-GGamma13-GFP2

   Plasmid

   #140992

   Purpose

   Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma13-GFP2 (GNG13 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Hyg-RFPKDEL

    Plasmid

    #138660

    Purpose

    Reporter plasmid expressing the ER-located RFP

    Depositor

    Bertrand Collet

    Article

    Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

    Insert

    RFP-KDEL
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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