Addgene

We narrowed to 151,104 results for: Des

Showing: 2801 - 2840 of 151104 results

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pET15b-mISG15(GG)

Plasmid

#12448

Depositor

Dong-Er Zhang

Article

Kim et al Methods Enzymol. 2005 . 398():491-9.

Insert

C-terminal processed form of ISG15

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pRS403-PGAL1-strongSC_GFP

Plasmid

#22316

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9-GFP

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pYES2.1-Dcr1

Plasmid

#22307

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

DCR1

Use

2micron

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pYES2.1-Ago1

Plasmid

#22306

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

AGO1

Use

2micron

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pME.E (pDHg16)

Plasmid

#24573

Depositor

David Pulleyblank

Article

Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

Insert

pME.E (pDHg16)

Use

Tags

Expression

Mutation

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pME.C (pDHf14)

Plasmid

#24572

Depositor

David Pulleyblank

Article

Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

Insert

Use

Tags

Expression

Mutation

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
pRS403-PGAL1-weakSC_GFP

Plasmid

#22315

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET101-Dcr1

Plasmid

#22309

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

DCR1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only
plp-strongSC_GFP

Plasmid

#22312

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9-GFP

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-ScDcr1

Plasmid

#32036

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

Drc1

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29_LuxSit_i

Plasmid

#194299

Purpose

Bacterial expression of the de novo luciferase, LuxSit-i

Depositor

Andy Yeh, David Baker

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

Plasmid

#198337

Purpose

PB plasmid expressing Halo-tagged PP7 coat protein

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

PP7 coat protein 3x Halo-GB1

Use

Piggybac

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hL1

Plasmid

#89411

Purpose

mammalian expression of hL1

Depositor

Fritz Rathjen

Article

De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

Insert

L1CAM (L1CAM Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

Plasmid

#121976

Purpose

transfection studies in eukaryotic cells such as COS-7 cells

Depositor

Christophe Erneux

Article

De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

Insert

Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCX-2A-2A

Plasmid

#74670

Purpose

based on pCX-C1 with F2A1/F2A2 inserted

Depositor

Roberto Giovannoni

Article

De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-3xNLS-OptoJNKi

Plasmid

#89740

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKCAG-mCherry-OptoJNKi

Plasmid

#89738

Purpose

Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-NES-OptoJNKi

Plasmid

#89739

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pluc-miR148a-148aT

Plasmid

#65053

Purpose

Reporter plasmid to detect miR148/152 family expression

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

Engineered target sites miR-148a-3p (MIR148A Mouse, Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SnoopTag

Plasmid

#225195

Purpose

Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SnoopTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAX1_HS∆P2

Plasmid

#225197

Purpose

Used to create a markerless deletion of the E. coli HS P2 phage

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

P2 phage homology arms

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAX1_HS_gpN:SpyTag003

Plasmid

#225194

Purpose

Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag003

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDsRed1-CMV-Rat Arc-DsRed1-pA

Plasmid

#233049

Purpose

To Express a Rat Arc-DsRed fusion protein from a CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

Insert

rat Arc-DsRed fusion (Arc Rat)

Use

Tags

DsRed1

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_4H.01

Plasmid

#155210

Purpose

Expresses 4H.01 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4H.01

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_4E2H.210

Plasmid

#155212

Purpose

Expresses 4E2H.210 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS405-PTEF-Dcr1

Plasmid

#22314

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

DCR1

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS404-PTEF-Ago1

Plasmid

#22313

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

AGO1

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS403-PGAL1-hpSC_URA3

Plasmid

#22317

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

URA hairpin

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT127-SEP

Plasmid

#41899

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Use

Tags

superecliptic GFP (SEP)

Expression

Yeast

Mutation

Promoter

Available since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGRB2.1

Plasmid

#45341

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Tags

Expression

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBM16

Plasmid

#45344

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSF-KpDcr1

Plasmid

#32022

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGRB2.0

Plasmid

#45340

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Tags

Expression

Mutation

Promoter

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBM16.1

Plasmid

#45345

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Tags

Expression

Mutation

Promoter

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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