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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,481 results for: D10

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Showing: 1401 - 1420 of 2481 results

  1. ABE8.13-d

    Plasmid
    #136297
    Purpose
    expresses ABE8.13-d in mammalian cells
    Depositor
    Nicole Gaudelli
    Publication
    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE8.13-d
    Tags
    C-terminal BPNLS
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    136297-plasmid-map-sequence-id-269928

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  5. pYPQAT34

    Plasmid
    #223406
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223406-plasmid-map-sequence-id-454165

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  11. pYPQAT25

    Plasmid
    #223397
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223397-plasmid-map-sequence-id-453300

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  14. pDEST27-GST-hADAM10

    Plasmid
    #189553
    Purpose
    Protein expression ADAM10 in mammalian cells.
    Depositor
    Haian Fu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    GST
    Expression
    Mammalian
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    189553-plasmid-map-sequence-id-384138

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  17. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid
    #227322
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227322-plasmid-map-sequence-id-452101

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  18. pafG-CBE-3T

    Plasmid
    #220374
    Purpose
    for hA3A-nCas9 and gRNA expression in Aspergillus flavus
    Depositor
    Xue Gao
    Publication
    Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    hA3A-nCas9
    tRNA and gRNA scaffold
    Use
    CRISPR
    Promoter
    Ptef1 and tRNA
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220374-plasmid-map-sequence-id-440033

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  19. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

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  20. mEos3.2trCD45

    Plasmid
    #203753
    Purpose
    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Publication
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    trCD45 (PTPRC Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203753-plasmid-map-sequence-id-403339

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Showing: 1401 - 1420 of 2481 results