We narrowed to 3,045 results for: ER;

1. pET SUMO-syt7 C2AB-2A

   Plasmid

   #213627

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 C2AB-2A (Syt7 Mouse)
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   I298, L361A

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSynW pHluorin-SYT9 IRES mRuby3

   Plasmid

   #195698

   Purpose

   Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFSynW Syt9 IRES mRuby3

   Plasmid

   #195700

   Purpose

   Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Mutation

   encodes only the first 68 amino acids of Tac1

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFSynW SYT9 D197, 199N IRES GFP

   Plasmid

   #195701

   Purpose

   Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Mutation

   full-length mouse SYT9 with D197, 199N mutations

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSynW SYT9 D330, 332N IRES GFP

   Plasmid

   #195702

   Purpose

   Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Mutation

   full-length mouse SYT9 with D330, 332N mutations

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX SYT9 C2AB D197,199,330,332N

   Plasmid

   #195706

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 104-386 of mouse SYT9 with D1…

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-ST-P2A-pSC-CAAX

   Plasmid

   #207637

   Purpose

   A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
   

   Expression

   Mammalian

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   CMV

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC-cUnaG-LPETG

   Plasmid

   #207635

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-Best1-Txnip(C247S_LL351-352AA)

   Plasmid

   #206344

   Purpose

   AAV plasmid expressing mutant TXNIP in retinal pigment epithelium

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

   Insert

   Txnip (Txnip Mouse)
   

   Use

   AAV

   Mutation

   C247S, L351A, L352A

   Promoter

   human Best1

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-dora[B]_rescue_construct

    Plasmid

    #190638

    Purpose

    Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-dora[A]_rescue_construct

    Plasmid

    #190640

    Purpose

    Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJS697

    Plasmid

    #168778

    Purpose

    Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Type

    Empty backbone

    Tags

    Cmyc and HA

    Expression

    Yeast

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-HisFlag-rUSP19ER-CAmut

    Plasmid

    #155237

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLz26_CsoS2_N1-N2_GFP

    Plasmid

    #140851

    Purpose

    His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeats 1-2 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111922

    Purpose

    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Ubl4A K48R

    Plasmid

    #61809

    Purpose

    expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    UBL4A (UBL4A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K48R

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pBS cSugarCrisp (CT#708)

    Plasmid

    #13956

    Depositor

    Cliff Tabin

    Article

    Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

    Insert

    SugarCrisp (PI15 Chicken)
    

    Expression

    Bacterial

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only