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Showing: 1401 - 1420 of 8185 results

  1. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  6. pBAF

    Plasmid
    #104152
    Purpose
    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.
    Depositor
    Robert Craigie
    Article
    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95
    Insert
    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    Use
    Tags
    N-terminal His tag with thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    104152-plasmid-map-sequence-id-201395

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  8. pSLC-237

    Plasmid
    #73191
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    ParaB-relE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ParaB
    Available since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    73191-plasmid-map-sequence-id-151362

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  10. pHUC-JEDI-1P

    Plasmid
    #202619
    Purpose
    Genetically encoded voltage indicator (GEVI) JEDI-1P under the HuC promoter for pan neuronal expression in zebrafish; JEDI-1P sequence codon-optimized for zebrafish expression
    Depositor
    Francois St-Pierre
    Article
    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:
    Insert
    JEDI-1P
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    HuC
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    202619-plasmid-map-sequence-id-398002

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  13. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  15. SpyTag-β-Lactamase-SpyCatcher

    Plasmid
    #52656
    Purpose
    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.
    Depositor
    Mark Howarth
    Article
    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2
    Insert
    TEM-1 β-Lactamase
    Use
    Tags
    SpyCatcher and SpyTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52656-plasmid-map-sequence-id-422741

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  18. pV1F

    Plasmid
    #205084
    Purpose
    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.
    Depositor
    Suomeng Dong
    Article
    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.
    Insert
    AtVIP1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    agorbacterium virB operon promoter
    Available since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    205084-plasmid-map-sequence-id-405743

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  19. pSLC-246

    Plasmid
    #73190
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-tse2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73190-plasmid-map-sequence-id-151367

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Showing: 1401 - 1420 of 8185 results