We narrowed to 1,413 results for: Flp

1. AiP981-pAAV-mscRE4-minBGpromoter-EGFP-WPRE-hGHpA

   Plasmid

   #163484

   Purpose

   Direct-expressing EGFP AAV Virus. Alias: AiP981 - pAAV-AiE2004m-minBG-EGFP-WPRE-HGHpA

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   Beta Globin minimal promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

   Plasmid

   #129421

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-3Flag
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   3X Flag epitope tag

   Promoter

   none (3X polyA to mitigate episomal expression)

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA

   Plasmid

   #163476

   Purpose

   Direct-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpA

   Depositor

   Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   iCre
   

   Use

   AAV

   Promoter

   Beta Globin minimal promoter

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

   Plasmid

   #67277

   Purpose

   inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

   Inserts

   first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
   second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
   

   Tags

   mTQ2 blue fluorescent and red flourescent gene ka…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MADR pDonor smFP-HA (dark) WPRE

   Plasmid

   #131007

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-HA WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   HA - 10 total HA epitope tages

   Expression

   Mammalian

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MADR pDonor smFP-V5 (dark) WPRE

   Plasmid

   #131006

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   V5 - 10 total of V5 epitope tag

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MADR pDonor smFP-Myc (dark) WPRE

   Plasmid

   #130987

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-Myc WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   Myc - 10 total Myc tags

   Mutation

   "dark" variant with GGG fluorophore ver…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MADR pDonor smFP-FLAG (dark) WPRE

   Plasmid

   #131005

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-FLAG WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   FLAG - 10 total FLAG tags

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131462

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A K27M, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131461

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131463

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A G34R, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid

    #129420

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    TagBFP2-V5 tag

    Mutation

    G12V

    Promoter

    none (3x polyA to mitigate episomal expression)

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid

    #131730

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    TagBFP2-V5 tag

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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