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BMPR1A gRNA (BRDN0001147772)

Plasmid

#77960

Purpose

3rd generation lentiviral gRNA plasmid targeting human BMPR1A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BMPR1A (BMPR1A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

Plasmid

#171101

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Olig2 Mouse, S. pyogenes)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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GNE gRNA (BRDN0001145959)

Plasmid

#77832

Purpose

3rd generation lentiviral gRNA plasmid targeting human GNE

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GNE (GNE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MELK gRNA (BRDN0001146713)

Plasmid

#75669

Purpose

3rd generation lentiviral gRNA plasmid targeting human MELK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MELK (MELK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IKBKE gRNA (BRDN0001147958)

Plasmid

#77770

Purpose

3rd generation lentiviral gRNA plasmid targeting human IKBKE

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IKBKE (IKBKE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALPK2 gRNA (BRDN0001148059)

Plasmid

#77521

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALPK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALPK2 (ALPK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALPK2 gRNA (BRDN0001147473)

Plasmid

#77523

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALPK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALPK2 (ALPK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TKFC gRNA (BRDN0001144729)

Plasmid

#75783

Purpose

3rd generation lentiviral gRNA plasmid targeting human TKFC

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TKFC (DAK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK3 gRNA (BRDN0001149001)

Plasmid

#75548

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK3 (DYRK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HIPK3 gRNA (BRDN0001146408)

Plasmid

#77032

Purpose

3rd generation lentiviral gRNA plasmid targeting human HIPK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HIPK3 (HIPK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GSG2 gRNA (BRDN0001146968)

Plasmid

#77519

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSG2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSG2 (HASPIN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK19 gRNA (BRDN0001149215)

Plasmid

#76502

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK19

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK19 (CDK19 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K7 gRNA (BRDN0001162319)

Plasmid

#77821

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LMPd Amt PHGDH

Plasmid

#209409

Purpose

Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al Immunity. 2019 Nov 19;51(5):856-870.e5.

Insert

Phgdh shRNA (Phgdh Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ETS2_5-2)-PGKpuroBFP-W

Plasmid

#211958

Purpose

Express gRNA against ETS2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ETS2 (ETS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ARID2(14))-PGKpuro2ABFP-W

Plasmid

#200472

Purpose

Lentiviral vector expressing gRNA targeting human ARID2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID2(14) (ARID2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

Plasmid

#209197

Purpose

Mutagenesis of Faah

Depositor

Larry Zweifel

Article

Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

Insert

Faah (Faah Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFlt4#1/Cre

Plasmid

#193215

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Flt4 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgFlt4#1 (Flt4 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

Plasmid

#129034

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA6 (huTSDR)

Use

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

Plasmid

#91883

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only