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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,574 results for: golden gate

Showing: 1401 - 1420 of 3574 results

  5. pAIP4_4IPC

    Plasmid
    #234229
    Purpose
    Heterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4IPC (RPA1 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234229-plasmid-map-sequence-id-464194

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  6. pAIP4_4QMD

    Plasmid
    #234174
    Purpose
    Heterologous protein expression of envoplakin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QMD (EVPL Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234174-plasmid-map-sequence-id-464139

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  7. pAIP4_1BKR

    Plasmid
    #234168
    Purpose
    Heterologous protein expression of spectrin beta chain, non-erythrocytic 1 (Beta-II spectrin) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1BKR (SPTBN1 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234168-plasmid-map-sequence-id-464134

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  8. pAIP4_2JIK

    Plasmid
    #234170
    Purpose
    Heterologous protein expression of synaptojanin-2-binding protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2JIK (SYNJ2BP Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234170-plasmid-map-sequence-id-464136

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  9. pAIP4_3RZY

    Plasmid
    #234156
    Purpose
    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3RZY (FABP4 Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234156-plasmid-map-sequence-id-464122

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  10. pAIP4_5EF9

    Plasmid
    #234154
    Purpose
    Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5EF9 (PB2 Influenza B virus (B/Lee/1940))
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234154-plasmid-map-sequence-id-464120

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  16. pMulti_K_ccdB

    Plasmid
    #223852
    Purpose
    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)
    Depositor
    Stephan Gruber
    Article
    Taschner et al bioRxiv 2024.06.17.599261
    Type
    Empty backbone
    Use
    Golden-gate acceptor
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    223852-plasmid-map-sequence-id-456126

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  17. pMulti_S_ccdB

    Plasmid
    #223853
    Purpose
    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)
    Depositor
    Stephan Gruber
    Article
    Taschner et al bioRxiv 2024.06.17.599261
    Type
    Empty backbone
    Use
    Golden-gate acceptor
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    223853-plasmid-map-sequence-id-454892

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  18. pMulti_A_ccdB

    Plasmid
    #223851
    Purpose
    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)
    Depositor
    Stephan Gruber
    Article
    Taschner et al bioRxiv 2024.06.17.599261
    Type
    Empty backbone
    Use
    Golden-gate acceptor
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    223851-plasmid-map-sequence-id-450699

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  19. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

    Plasmid
    #219701
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219701-plasmid-map-sequence-id-435731

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  20. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

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Showing: 1401 - 1420 of 3574 results