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We narrowed to 3,800 results for: golden gate

Showing: 1401 - 1420 of 3800 results

  1. pMV_AA097

    Plasmid
    #245319
    Purpose
    Cas12a CRISPRko positive control; targets CD46, CD47, CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46, CD47, CD55 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245319-plasmid-map-sequence-id-488024

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  2. pDest_PANCEd_AraC_R2_LDelK

    Plasmid
    #242156
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE/RAMPhaGE Linker Library variant cassette
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV LDelK
    Expression
    Bacterial
    Mutation
    AraC: E3K, R146L, A152T, R251L; Left Linker: GS -…
    Available Since
    Sept. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    242156-plasmid-map-sequence-id-486033

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  3. pAttBFHyg

    Plasmid
    #244926
    Purpose
    attB Hyg Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244926-plasmid-map-sequence-id-486007

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  4. pAIP4_6WEY

    Plasmid
    #234234
    Purpose
    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234234-plasmid-map-sequence-id-464198

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  5. pAIP4_3D1B

    Plasmid
    #234230
    Purpose
    Heterologous protein expression of RNA-induced transcriptional silencing complex protein tas3 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3D1B (tas3 Fission Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234230-plasmid-map-sequence-id-464195

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  6. pAIP4_5F66

    Plasmid
    #234226
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5F66 (PPIA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234226-plasmid-map-sequence-id-464191

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  7. pAIP4_5HIH

    Plasmid
    #234205
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234205-plasmid-map-sequence-id-464170

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  8. pAIP4_5EIP

    Plasmid
    #234188
    Purpose
    Heterologous protein expression of RNA binding exosome specificity factor Mmi1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5EIP (mmi1 Fission Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234188-plasmid-map-sequence-id-464153

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  9. pAIP4_5KUW

    Plasmid
    #234183
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5KUW (PPIA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234183-plasmid-map-sequence-id-464148

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  10. pAIP4_3ONH

    Plasmid
    #234179
    Purpose
    Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ONH (UBA2 Budding Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234179-plasmid-map-sequence-id-464144

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  11. pAIP4_1HKA

    Plasmid
    #234169
    Purpose
    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1HKA (folK Escherichia coli)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234169-plasmid-map-sequence-id-464135

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  12. pAIP4_6BIO

    Plasmid
    #234152
    Purpose
    Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6BIO (TVAG_298080 Trichomonas vaginalis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234152-plasmid-map-sequence-id-464118

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  13. pAIP4_2H2Z

    Plasmid
    #234147
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234147-plasmid-map-sequence-id-464113

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  14. L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10

    Plasmid
    #219699
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219699-plasmid-map-sequence-id-435730

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  15. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

    Plasmid
    #219689
    Purpose
    Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB (mtagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219689-plasmid-map-sequence-id-435724

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  16. pJ1-g1g2-K2

    Plasmid
    #218217
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatible
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218217-plasmid-map-sequence-id-438339

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  17. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

    Plasmid
    #219711
    Purpose
    Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219711-plasmid-map-sequence-id-435738

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  18. MUT_S1-N

    Plasmid
    #218398
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218398-plasmid-map-sequence-id-433922

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  19. MUT_N-H1

    Plasmid
    #218397
    Purpose
    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218397-plasmid-map-sequence-id-433918

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  20. pK7-g7g8-K8

    Plasmid
    #218220
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218220-plasmid-map-sequence-id-432226

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Showing: 1401 - 1420 of 3800 results