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  4. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid
    #169737
    Purpose
    Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169737-plasmid-map-sequence-id-334143

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  18. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

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Showing: 1401 - 1420 of 1508 results