We narrowed to 2,454 results for: puc19

Showing: 1401 - 1420 of 2454 results

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qTAG-C-moxGFP-Blast

Plasmid

#207710

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Aurli_150841_GZG

Plasmid

#162563

Purpose

Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

Depositor

Jackie Collier

Article

Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

Insert

shble

Use

Unspecified

Promoter

GAPDH

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAcCAST-sgRNA_entry (CJT83)

Plasmid

#181785

Purpose

Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHBJT001

Plasmid

#225152

Purpose

High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pADH143

Plasmid

#90989

Purpose

NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pADH140

Plasmid

#90988

Purpose

NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

C. maltosa LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mNeon-Zeo

Plasmid

#207731

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Puro

Plasmid

#207724

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-mNeon

Plasmid

#207698

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-V5

Plasmid

#207697

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pShoCAST-sgRNA_entry (BO1)

Plasmid

#181786

Purpose

Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-moxGFP

Plasmid

#207688

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCRISPR_full-site

Plasmid

#186277

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xHA-Zeo

Plasmid

#207740

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-3xHA

Plasmid

#207707

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSZT025

Plasmid

#140033

Purpose

KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Expression

Bacterial

Promoter

cLac143

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCR1075

Plasmid

#111093

Purpose

Expresses eGFP donor in pUC19

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

eGFP

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pADH139

Plasmid

#90987

Purpose

NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

gRNA conserved, C, mal LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miniTurbo-Zeo

Plasmid

#207736

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Zeo-miniTurbo

Plasmid

#207703

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge