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Showing: 1401 - 1420 of 1683 results

  5. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112078-plasmid-map-sequence-id-217613

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  6. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112076-plasmid-map-sequence-id-217621

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  7. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  8. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  10. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  13. miniABEmax (pRZ900)

    Plasmid
    #131311
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131311-plasmid-map-sequence-id-254652

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  14. pET28b-BE3∆UGI

    Plasmid
    #96943
    Purpose
    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).
    Depositor
    Daesik Kim
    Article
    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.
    Insert
    BE3∆UGI
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96943-plasmid-map-sequence-id-193667

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  15. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid
    #131313
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    131313-plasmid-map-sequence-id-254655

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  16. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112073-plasmid-map-sequence-id-217642

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  17. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130923-plasmid-map-sequence-id-257307

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  18. pLY9

    Plasmid
    #130907
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-2G6, and Ptet
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    130907-plasmid-map-sequence-id-258306

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  19. pLY59

    Plasmid
    #130927
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    Use
    Synthetic Biology
    Tags
    ASV tag and MCP (MS2 coat protein)
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-2G6, and Ptet
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130927-plasmid-map-sequence-id-257328

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  20. pLY57

    Plasmid
    #130926
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130926-plasmid-map-sequence-id-257327

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Showing: 1401 - 1420 of 1683 results