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phROSA26-Tet-HA-JSAP1_WT

Plasmid

#208772

Purpose

Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JSAP1 (MAPK8IP3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRK172-α-syn-TEV-GFP

Plasmid

#166671

Purpose

Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

Depositor

Iris Lindberg

Article

Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

Insert

mouse synuclein (from V. Lee) (Snca Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

added a TEV cleavage site sequence between synucl…

Promoter

CMV

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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EYFP-p65

Plasmid

#111192

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m

Plasmid

#192274

Purpose

Encodes MmCas12m under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 proHB-EGF CS

Plasmid

#11601

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Use

Tags

His and myc

Expression

Mammalian

Mutation

Constitutively secreted HB-EGF: C-terminal amino …

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-GATA2

Plasmid

#125028

Purpose

doxycycline-inducible expression of human GATA2 in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

GATA binding protein 2 (GATA2 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hcMYC

Plasmid

#206387

Purpose

Expresses human c-MYC in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

c-MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Aa

Plasmid

#128331

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform a

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Ab

Plasmid

#128332

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform b

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-GFI1B

Plasmid

#125597

Purpose

doxycycline-inducible expression of human GFI1B in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

Growth Factor Independent 1B transcriptional repressor (GFI1B Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-Flag-IKKbeta

Plasmid

#15465

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
Luciferase

Plasmid

#213979

Purpose

Expresses Doxy Inducible Luciferase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Luciferase

Use

Lentiviral

Tags

6xHis affinity tag

Expression

Bacterial and Mammalian

Mutation

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX17FNV

Plasmid

#206384

Purpose

Expresses human SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX17FNV (SOX17 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-HA-IKKbeta

Plasmid

#15470

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pEMS2113

Plasmid

#49138

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

FEV

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pVAX1-BMP2/7 +

Plasmid

#137911

Purpose

Constitutive mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, tandem)

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Inserts

BMP2 (BMP2 Human)
BMP7 (BMP7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTarget: Ptaq-RFP_PlacIq-GFP

Plasmid

#192280

Purpose

RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Inserts

RFP
GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacIq and Ptaq

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1991

Plasmid

#49122

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-iCre-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

FEV

Available since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-tetO-HA-GFI1B

Plasmid

#125599

Purpose

doxycycline-inducible expression of human GFI1B (HA tagged) in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

Growth Factor Independent 1B transcriptional repressor (GFI1B Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

Plasmid

#84244

Purpose

Expresses GA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sa dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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