We narrowed to 7,373 results for: Trac

1. pLBH545_Tet-Cas14a1_Locus

   Plasmid

   #112501

   Purpose

   Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. LgBit-NbALFA

   Plasmid

   #201484

   Purpose

   NanoBit detection of ALFA-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   LgBit-NbALFA
   

   Use

   Tags

   LgBit

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.Syn.MTRIA-OT

   Plasmid

   #184594

   Purpose

   For production of AAV for a fluorescent sensor for oxytocin

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-OT
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Human synapsin promoter

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. DL033:cFos::eGFP-iPAK4

   Plasmid

   #177883

   Purpose

   eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   cFos

   Available Since

   July 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Myc-GFP-Vpr-RR

   Plasmid

   #83374

   Purpose

   Expression of Myc tagged GFP-Vpr fusion protein

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   Myc-GFP-Vpr
   

   Use

   Tags

   Myc and Myc-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR322-Pdp1-HiBiT

   Plasmid

   #198310

   Purpose

   Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA HA FAK Y397F

   Plasmid

   #50503

   Depositor

   Kenneth Yamada

   Article

   Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y397F

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

   Plasmid

   #92400

   Purpose

   Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

   Depositor

   Periklis Pantazis

   Article

   Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

   Inserts

   Dendra2
   Cerulean
   

   Use

   Zebrafish expression

   Tags

   H2B tag and membrane tag

   Expression

   Mutation

   Promoter

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVL SFFV-EF1s HA.NLS.Sce(opt)

   Plasmid

   #31479

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV/Ef1s HA NLS I-SceI
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   "opt" indicates the coding sequence has…

   Promoter

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP

    Plasmid

    #24033

    Depositor

    Cori Bargmann

    Article

    Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

    Insert

    Npr1 (npr-1 Nematode)
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. LT1_33_pTEFmut8-mCherry

    Plasmid

    #177706

    Purpose

    Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEFmut8-mCherry
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. betaRBPIG

    Plasmid

    #16422

    Depositor

    Juergen Hescheler

    Article

    Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

    Insert

    loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pCL28-decoder

    Plasmid

    #159450

    Purpose

    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    PlexAtet-LexA-ER-B112-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PlexAtet

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCL66-decoder

    Plasmid

    #159453

    Purpose

    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1mut-tetR-nls-malE-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfus1mut

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TS246_pNKI-2-13_HALO-DPN7

    Plasmid

    #200720

    Purpose

    producing Methyl Tracer HALO protein in bacteria

    Depositor

    Bas van Steensel

    Article

    van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

    Insert

    HALO-DPN7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEYFP-p23

    Plasmid

    #108225

    Purpose

    Mammalian expression vector for EYFP fusion to human p23

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EYFP-p23 (PTGES3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pN-PITCh-GFP

    Plasmid

    #127888

    Purpose

    Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    Puro-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIS.MTRIA-ATP

    Plasmid

    #184599

    Purpose

    For expression of a fluorescent sensor for ATP in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEmerald-Vinexin-C-14

    Plasmid

    #54305

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Vinexin (SORBS3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. Actinin-sstFRET-GR

    Plasmid

    #83416

    Purpose

    FRET-based actinin tension sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    Actinin-sstFRET-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only