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pKmK.I4R

Plasmid

#125066

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Publication

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome IV:240042..241741

Use

Synthetic Biology

Expression

Yeast

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I4L

Plasmid

#125033

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Publication

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to chromosome IV:240042..241741

Use

Synthetic Biology

Expression

Yeast

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL13001

Plasmid

#50265

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb) + 5'UTR, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCB036 FoMV-sgGFP

Plasmid

#234813

Purpose

To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

Depositor

Daniel Voytas

Publication

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting GFP

Expression

Plant

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE163

Plasmid

#118511

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTmpSm

Plasmid

#176228

Purpose

A template plasmid for amplification of the cI-hok-strA cassette

Depositor

Sergey Sineoky

Publication

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

cI of phage lambda, hok of the R1 plasmid, strA

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFG036

Plasmid

#137996

Purpose

cI repressor expression plasmid

Depositor

Sébastien Rodrigue

Publication

Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

Insert

cI

Use

Synthetic Biology

Expression

Bacterial

Mutation

Thermosensitive

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE167

Plasmid

#118515

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPI459

Plasmid

#113196

Purpose

extrachromosomal expression vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector, extra…

Tags

mScarlet

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

Plasmid

#174645

Purpose

Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes

Depositor

Lukas Kapitein

Publication

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11

Tags

SSPB(micro)-Venus and iLID-mCherry

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Expression

Worm

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-YFP

Plasmid

#74154

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Publication

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-YFP

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pICH78133

Plasmid

#50292

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Publication

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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A*02:02:01:01

Plasmid

#203277

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Publication

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

A*02:02:01:01 (HLA-A Human)

Expression

Mammalian

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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B*58:01:01

Plasmid

#203261

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Publication

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

B*58:01:01 (HLA-B Human)

Expression

Mammalian

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Publication

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Expression

Insect

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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