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pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

Plasmid

#204579

Purpose

lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

mCherry

Use

Lentiviral

Promoter

TRE3G

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-tTA

Plasmid

#99119

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

hSyn1

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP5

Plasmid

#220071

Purpose

overexpresses ORP5 with a neonGreen fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5 (OSBPL5 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_Thy1StTA

Plasmid

#97411

Purpose

TET driver for amplified expression in cortical neurons

Depositor

Tetsuo Yamamori

Article

Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

Insert

tTA

Use

AAV

Expression

Mammalian

Promoter

mouse thy1PSs

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHR myc-MBP-SspB-iRFP-NLS

Plasmid

#223286

Purpose

An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

Myc-MBP-SspB-irFP-NLS

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Gi3-CASE

Plasmid

#168122

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)

Use

Luciferase

Tags

cpVenus on GNG2

Expression

Mammalian

Mutation

NLuc is inserted at A114/E115 within GNAI1

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAIL-R2

Plasmid

#61383

Purpose

Expresses TRAIL receptor 2

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 2 (TNFRSF10B Human)

Tags

none

Expression

Mammalian

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry

Plasmid

#41583

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Has Service

Cloning Grade DNA

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pIVT-dCas9-mCherry

Plasmid

#174443

Purpose

in vitro transcription template for dCas9-mCherry

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

mCherry

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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