165,652 results

1. MCP-mCherry

   Plasmid

   #207668

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP)

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   MCP-mCherry
   

   Expression

   Mammalian

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-TripleColour-StAR

   Plasmid

   #222695

   Purpose

   Competition assay

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP, miRFP703, dTomato

   Expression

   Mammalian

   Mutation

   PGK, TagBFP, dTomato without BsmBI cutsite

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

   Plasmid

   #197510

   Purpose

   Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and NpuN(intein)-BPNLS

   Mutation

   ABE8e mutations in TadA and nSpRY(D10A/A61R)

   Promoter

   Cbh

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiFCt-2in1-CC

   Plasmid

   #105114

   Purpose

   ratiometric BiFC analysis in plants (transient transformation)

   Depositor

   Christopher Grefen

   Article

   Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

   Type

   Empty backbone

   Tags

   cYFP-Myc/nYFP-HA

   Expression

   Plant

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE-60 roGFP2-Orp1-His

   Plasmid

   #64976

   Purpose

   Bacterial expression of His-tagged roGFP2-Orp1

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Tags

   His and roGFP2

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6.3/EV

   Plasmid

   #120848

   Purpose

   Control vector, 20nt random sequence between gateway recombination sites

   Depositor

   Jan Rehwinkel

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-32a(+)-NRG1B1

   Plasmid

   #120287

   Purpose

   Produce human recombinant NRG1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   NRG1-beta 1
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR89

    Plasmid

    #140096

    Purpose

    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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