We narrowed to 14,584 results for: pET
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Plasmid#197000PurposeBacterial expression plasmid for gamma toxin tRNA endonucleaseDepositorInsertGamma toxin
UseTagsHis10-Smt3ExpressionBacterialMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp43(exo-)
Plasmid#228202PurposeExpression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219ADepositorInsert43
UseTagsSce VMA intein-chitin binding domainExpressionBacterialMutationMutated aspartate 219 to alanine (D219A), elimina…PromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos36)GFP
Plasmid#62937PurposeExpression of 6xHis-(+36)GFP in bacterial cellsDepositorInsert(pos36)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ACPs (from S. pneumonia)
Plasmid#63687PurposeInducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniaeDepositorInsertacpS
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-ARH3-His-Sumo-HA
Plasmid#111578PurposeExpresses ARH3 (amino acids 14-363) in E. coliDepositorInsertARH3 (ADPRS Human)
UseTags6xHis-Sumo and HAExpressionBacterialMutationdeleted amino acids 1-13PromoterT7Available sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RnlA-Strep
Plasmid#159350PurposeExpress TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectivelyDepositorInsertTS2126 RNA ligase
UseTagsHis6 and StrepExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET21a-GFP-AURKA_K162M-mCherry
Plasmid#99881PurposeExpression of GFP-AURKA K162M-mCherry for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFP and mCherryExpressionBacterialMutationK162M (kinase-dead)PromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry-Cbx5
Plasmid#194547PurposeBacterial Expression of 6xHis mCherry-HP1a Fusion ProteinDepositorInsertCbx9 (HP1a)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
UseTags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP-HMGB1-WT
Plasmid#194543PurposeBacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
UseTags10xHis-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp10
Plasmid#154959PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-21a(+)-AK1-His
Plasmid#118977PurposeBacterial expression plasmid for Adenylate kinase isoenzyme 1DepositorInsertadenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos14)GFP
Plasmid#89252PurposeExpresses GFP with +14 theoretical charge in E.ColiDepositorInsertHis6-(pos16)-GFP
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos15)GFP
Plasmid#89248Purpose[Edit] Expresses GFP with +15 theoretical charge in E.ColiDepositorInsertHis6-(pos15)-GFP
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
UseTags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389PromoterAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mOct4 Full length(FL)
Plasmid#206394PurposeExpresses Oct4 with a 6xHis tag for protein purificationDepositorInsertOct4 (Pou5f1 Mouse)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry-NPM1
Plasmid#194546PurposeBacterial Expression of 6xHis mCherry-NPM1 Fusion ProteinDepositorInsertNPM1 (Npm1 Mouse)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP2 (catalytic domain)
Plasmid#16357DepositorInserthuman SENP2 catalytic domain (SENP2 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 365 to 590 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-42)
Plasmid#127151PurposeExpresses N-terminal cysteine Abeta(1-42)DepositorInsertAbeta(MC1-42) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET23b-2E2-HA-mEGFP-6xHis
Plasmid#129594PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHaloTag141/145
Plasmid#194660PurposeProtein production of circular permuted HaloTag (cpHaloTag141/145) in bacteriaDepositorInsertcpHaloTag141/145
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHaloTag154/156
Plasmid#194661PurposeProtein production of circular permuted HaloTag (cpHaloTag154/156) in bacteriaDepositorInsertcpHaloTag154/156
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6His-MBP-MpAgo
Plasmid#79249PurposeThis plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expressionDepositorInsertMarinitoga piezophila Argonaute
UseCRISPRTags6XHis-tag + MBP + PreScission Cleavage SiteExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PET-WT SpCas9-NLS-6xHis
Plasmid#207373PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertWT SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-EIN-AlkBH5(66-292)
Plasmid#125732PurposePlasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.DepositorInsertAlkBH5(66-292) (ALKBH5 Human)
UseTags6xHis and EINExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-22b DT 51E/148K
Plasmid#11081DepositorInsertDT
UseTagsHisExpressionBacterialMutation51E/148K, catalytically inactivePromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp16_SARS-CoV-2
Plasmid#167850PurposeBacterial expression of the SARS-CoV-2 protein nsp16DepositorInsertnsp16
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-MHL: nsp10_SARS-CoV-2
Plasmid#167851PurposeBacterial expression of the SARS-CoV-2 protein nsp10DepositorInsertnsp10
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a-His6-ABP-LOXCAT
Plasmid#139971PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-K128N
Plasmid#120284PurposeProduce human recombinant FGF2 K128N in DE3 cellsDepositorInsertBasic fibroblast growth factor 2 (FGF2 Human, Synthetic)
UseTags6x HisExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP1 (catalytic domain)
Plasmid#16356DepositorInserthuman SENP1 catalytic domain (SENP1 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 419 to 644 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP7 (catalytic domain)
Plasmid#16360DepositorInserthuman SENP7 catalytic domain (SENP7 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 640 to 984 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-1-NONO(53-312)
Plasmid#135442PurposeExpresses NONO 53-312 in the second site; for co-expression with His-tagged proteinDepositorInsertNon-POU domain-containing octamer-binding protein (NONO Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-T7-NlaCas3-NLS-6xHis
Plasmid#180214PurposeExpressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.ColiDepositorInsertNlaCas3c
UseTags6xHis and NLSExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-Caspase-4_enzymatic-domain
Plasmid#183381PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only