Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,154 results for: FRI

Showing: 1441 - 1460 of 3154 results

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pcDNA FZD4_3linker

Plasmid

#184256

Purpose

Expresses FLAG tagged FZD4 swapped with FZD3 linker domain

Depositor

Hee-Jung Choi

Article

Ko et al Commun Biol. 2022 May 5;5(1):421. doi: 1

Insert

FZD4_3linker (Fzd4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Replaced FZD4 aa160-203 with FZD3 aa136-188

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FZD4_5linker

Plasmid

#184257

Purpose

Expresses FLAG tagged FZD4 swapped with FZD5 linker domain

Depositor

Hee-Jung Choi

Article

Ko et al Commun Biol. 2022 May 5;5(1):421. doi: 1

Insert

FZD4_5linker (Fzd4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Replaced FZD4 aa160-203 with FZD5 aa150-221

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FZD4_6linker

Plasmid

#184258

Purpose

Expresses FLAG tagged FZD4 swapped with FZD6 linker domain

Depositor

Hee-Jung Choi

Article

Ko et al Commun Biol. 2022 May 5;5(1):421. doi: 1

Insert

FZD4_6linker (Fzd4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Replaced FZD4 aa160-203 with FZD6 aa132-184

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FZD4_3CRDlinker

Plasmid

#184259

Purpose

Expresses FLAG tagged FZD4 swapped with FZD3 CRDlinker domain

Depositor

Hee-Jung Choi

Article

Ko et al Commun Biol. 2022 May 5;5(1):421. doi: 1

Insert

FZD4_3CRDlinker (Fzd4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Replaced FZD4 aa41-203 with FZD3 aa23-188

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FZD4_5CRDlinker

Plasmid

#184260

Purpose

Expresses FLAG tagged FZD5 swapped with FZD5 CRDlinker domain

Depositor

Hee-Jung Choi

Article

Ko et al Commun Biol. 2022 May 5;5(1):421. doi: 1

Insert

FZD4_5CRDlinker (Fzd4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Replaced FZD4 aa41-203 with FZD5 aa27-221

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FZD4_6CRDlinker

Plasmid

#184261

Purpose

Expresses FLAG tagged FZD4 swapped with FZD6 CRDlinker domain

Depositor

Hee-Jung Choi

Article

Ko et al Commun Biol. 2022 May 5;5(1):421. doi: 1

Insert

FZD4_6CRDlinker (Fzd4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Replaced FZD4 aa41-203 with FZD6 aa19-184

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-DHFR-3xFLAG

Plasmid

#233892

Purpose

lentiviral overexpression vector for DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMTHFD1

Plasmid

#217436

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting MTHFD1 (MTHFD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-TK2-3xFLAG

Plasmid

#217427

Purpose

Lentiviral overexpression of human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

TK2 (TK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTYMS

Plasmid

#217430

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_4

Plasmid

#217431

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 4 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_5

Plasmid

#217432

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 5 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgSHMT2

Plasmid

#217435

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting SHMT2 (SHMT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-FIVAR-ELP-HIS-ybbR

Plasmid

#169136

Purpose

E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

FIVAR (found in various architectures)

Tags

3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-Opto-Alk3

Plasmid

#207614

Purpose

Zebrafish Alk3 BMP receptor kinase domain fused to VfLOV

Depositor

Patrick Müller

Article

Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

Insert

Alk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Zebrafish, Vaucheria frigida)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-siRNAres_I

Plasmid

#146589

Purpose

Mammalian Expression of HsTNRC6A-siRNAres

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6A-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_1-1248_H

Plasmid

#146507

Purpose

Mammalian Expression of HsTNRC6A_1-1248

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

HsTNRC6A_1-1248 (TNRC6A Human)

Expression

Mammalian

Mutation

254-1962 truncated version of the sequence given …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F2V2-siRNAres_F

Plasmid

#146330

Purpose

Insect Expression of DmAGO1-F2V2

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmAGO1-F2V2 (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F629V-siRNAres_F

Plasmid

#146332

Purpose

Insect Expression of DmAGO1-F629V

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmAGO1-F629V (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F594V-siRNAres_F

Plasmid

#146331

Purpose

Insect Expression of DmAGO1-F594V

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmAGO1-F594V (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Showing: 1441 - 1460 of 3154 results

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