We narrowed to 2,341 results for: PUC19

1. pDiySpike3

   Plasmid

   #136950

   Purpose

   Plasmid for the In Vitro transcription of DiySpike3

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike3
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDiySpike1d1

   Plasmid

   #136951

   Purpose

   Plasmid for the In Vitro transcription of DiySpike1d1

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike1d1
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDiySpike1d2

   Plasmid

   #136952

   Purpose

   Plasmid for the In Vitro transcription of DiySpike1d2

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike1d2
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDiySpike1d3

   Plasmid

   #136953

   Purpose

   Plasmid for the In Vitro transcription of DiySpike1d3

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike1d3
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDiySpike1d4

   Plasmid

   #136954

   Purpose

   Plasmid for the In Vitro transcription of DiySpike1d4

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike1d4
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDiySpike2d1

   Plasmid

   #136955

   Purpose

   Plasmid for the In Vitro transcription of DiySpike2d1

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike2d1
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDiySpike2d2

   Plasmid

   #136956

   Purpose

   Plasmid for the In Vitro transcription of DiySpike2d2

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike2d2
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDiySpike2d3

   Plasmid

   #136957

   Purpose

   Plasmid for the In Vitro transcription of DiySpike2d3

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike2d3
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDiySpike2d4

   Plasmid

   #136958

   Purpose

   Plasmid for the In Vitro transcription of DiySpike2d4

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike2d4
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AP553‐1

    Plasmid

    #87240

    Purpose

    gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

    Insert

    gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
    

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFPB6

    Plasmid

    #83557

    Purpose

    Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL10 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas28

    Plasmid

    #82385

    Purpose

    sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23108

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas27

    Plasmid

    #82384

    Purpose

    sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23117

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAD06 K. lactis telomerase RNA hairpin

    Plasmid

    #61895

    Purpose

    Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin (nt 229-273)
    

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1_Puro_hPGK1_EGFP_Donor

    Plasmid

    #178088

    Purpose

    HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-AAVS1-PGK-Zeo

    Plasmid

    #227271

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-mStayGold-EFS-Puro

    Plasmid

    #227256

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Cas12i2-T2A-EGFP

    Plasmid

    #188270

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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