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pGEX-PML B-box1 WT

Plasmid

#185632

Purpose

expression of GST-tagged wild-type PML B-box1 domain in bacterial cells

Depositor

Irina Bezsonova

Publication

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

none

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-UnaG-FLAG

Plasmid

#203479

Purpose

For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG-FLAG

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCHSU74.2-2.2

Plasmid

#115543

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7

Expression

Yeast

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(D77R/T214V)

Plasmid

#66138

Purpose

Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Publication

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Gm1

Plasmid

#53528

Purpose

Expresses a plant arginyl-tRNA synthetase in E.coli

Depositor

Gabor Igloi

Publication

Igloi et al FEBS J. 2009 Mar;276(5):1307-18. doi: 10

Insert

arginyl-tRNA synthetase

Tags

His Tag and Thioredoxin

Expression

Bacterial

Promoter

T7

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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pL1L2 rtTA-FLAG (AJ13)

Plasmid

#257874

Purpose

Middle entry Gateway plasmid with FLAG-tagged Tet-On transactivator gene to make conditional transgenics

Depositor

Abigail Jensen

Publication

Campbell et al PLoS One. 2012;7(12):e51270. doi: 10.137

Insert

FLAG-tagged reverse Tet-On transactivator rtTA

Use

Gateway Cloning; Tet-on

Tags

FLAG

Promoter

none

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 C4C4

Plasmid

#185640

Purpose

expression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cells

Depositor

Irina Bezsonova

Publication

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

H155C H161C

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 W157E

Plasmid

#185641

Purpose

expression of GST-tagged mutant PML B-box1 W157E domain in bacterial cells

Depositor

Irina Bezsonova

Publication

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

W157E

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 F158E

Plasmid

#185642

Purpose

expression of GST-tagged mutant PML B-box1 F158E domain in bacterial cells

Depositor

Irina Bezsonova

Publication

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

F158E

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-tagRFP

Plasmid

#203482

Purpose

For Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

TagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-T136D-S15

Plasmid

#127824

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

T136D

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-S18

Plasmid

#127826

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Publication

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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p1G108CP20X

Plasmid

#85096

Purpose

Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Publication

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The G108C variant of PCNA1 fused to putidaredoxin

Tags

His6 tag

Expression

Bacterial

Mutation

G108C in PCNA1

Promoter

T7 promoter

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSOD1E133insTT-AcGFP1

Plasmid

#83441

Purpose

Expresses SOD1E133insTT in mammalian cells.

Depositor

Mohan Babu

Publication

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

E133insTT

Promoter

CMV

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-RPS8

Plasmid

#231492

Purpose

Expresses human ribosomal protein S8/eS8 with an N-terminal His-TrxA tag in bacterial cells

Depositor

Quira Zeidan

Publication

Nicholl et al MicroPubl Biol. 2026 Jul 9;2026:10.17912

Insert

RPS8 (RPS8 Human)

Tags

6xHis and Thioredoxin A

Expression

Bacterial

Promoter

T7

Available since

Sept. 11, 2026

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2_sg4_SYVN1

Plasmid

#257612

Purpose

CRISPR knockout of SYVN1 using sgRNA 4

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

sg4_SYVN1 (SYVN1 Human)

Use

Lentiviral

Promoter

U6

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2_sg2_SYVN1

Plasmid

#257611

Purpose

CRISPR knockout of SYVN1 using sgRNA 2

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

sg2_SYVN1 (SYVN1 Human)

Use

Lentiviral

Promoter

U6

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2_sg_Slc33a1

Plasmid

#257610

Purpose

CRISPR knockout of mouse Slc33a1

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

sg_Slc33a1 (Slc33a1 Mouse)

Use

Lentiviral

Promoter

U6

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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PMXS-OST4-mScarlet-3xHA-IRES-blasticidin

Plasmid

#255991

Purpose

PMXS construct expressing OST4-mScarlet-3xHA with an IRES-blasticidin cassette for ER-IP

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

OST4-mScarlet-3xHA (OST4 Human)

Use

Retroviral

Tags

mScarlet-3xHA

Expression

Mammalian

Mutation

N/A

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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PMXS-EMC3-mScarlet-3xMYC-IRES-bleomycin

Plasmid

#255988

Purpose

PMXS construct expressing EMC3-mScarlet-3xMYC with an IRES-bleomycin cassette for use as an IP control

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

EMC3-mScarlet-3xMYC (EMC3 Human)

Use

Retroviral

Tags

mScarlet-3xMYC

Expression

Mammalian

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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