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Showing: 1441 - 1460 of 1837 results

  1. pMT-HGP-v3-Nb127D01-hIgG

    Plasmid
    #171564
    Purpose
    Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells
    Depositor
    Norbert Perrimon, Hidde Ploegh
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
    Use
    Tags
    BiP signal peptide and hIgG1-hinge-N297A-TEV site…
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171564-plasmid-map-sequence-id-336182

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  4. pcDNA5 rtTA BirA eIF4G

    Plasmid
    #158789
    Purpose
    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1
    Use
    Tags
    3xFlag and BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    miniCMV
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158789-plasmid-map-sequence-id-309804

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  6. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

    Plasmid
    #226104
    Purpose
    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    COX8A (COX8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    encodes only for mitochondrial targeting sequence…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226104-plasmid-map-sequence-id-448409

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  7. pT7-EGFP-C1-HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres_W

    Plasmid
    #147897
    Purpose
    Mammalian Expression of HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres
    Depositor
    Elisa Izaurralde
    Article
    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:
    Insert
    HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    10 silent mutations and two non silent N605S, K75…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147897-plasmid-map-sequence-id-290344

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  10. pT7-EGFP-C1-HsNot1iso1_1-1605-del1079-1110-RNFtoAAA-shRNAres_W

    Plasmid
    #147886
    Purpose
    Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres
    Depositor
    Elisa Izaurralde
    Article
    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:
    Insert
    HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147886-plasmid-map-sequence-id-290333

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  11. pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W

    Plasmid
    #147887
    Purpose
    Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres
    Depositor
    Elisa Izaurralde
    Article
    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:
    Insert
    HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147887-plasmid-map-sequence-id-290334

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  12. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid
    #91820
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91820-plasmid-map-sequence-id-181105

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  13. RG6-BAP1_E7-D192D

    Plasmid
    #154024
    Purpose
    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)
    Depositor
    Samuel Peña-Llopis
    Article
    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:
    Insert
    BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
    Use
    Splicing reporter
    Tags
    DsRed1, FLAG, SV40 NLS, and eGFP
    Expression
    Mammalian
    Mutation
    BAP1 c.576C>T, p.D192D
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    154024-plasmid-map-sequence-id-307075

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  14. RG6-BAP1_E11-G312G

    Plasmid
    #154026
    Purpose
    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)
    Depositor
    Samuel Peña-Llopis
    Article
    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:
    Insert
    BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
    Use
    Splicing reporter
    Tags
    DsRed1, FLAG, SV40 NLS, and eGFP
    Expression
    Mammalian
    Mutation
    BAP1 c.936T>G, p.G312G
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    154026-plasmid-map-sequence-id-307080

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  16. pcDNA5 rtTA BirA eIF4G +MIC

    Plasmid
    #158790
    Purpose
    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon)
    Use
    Tags
    3xFlag and BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    miniCMV
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158790-plasmid-map-sequence-id-309812

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  17. pRcCMV-STAT31F

    Plasmid
    #134725
    Purpose
    Expresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707
    Depositor
    Curt Horvath, Jim Darnell
    Article
    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.
    Insert
    STAT3-STAT1 Fusion (STAT3, STAT1 Human)
    Use
    Tags
    FLAG and human STAT3 aa 1-514 joined to human STA…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    134725-plasmid-map-sequence-id-272781

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  18. pRcCMV-STAT13F

    Plasmid
    #134723
    Purpose
    Expresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707
    Depositor
    Curt Horvath, Jim Darnell
    Article
    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.
    Insert
    STAT1-STAT3 fusion (STAT3, STAT1 Human)
    Use
    Tags
    FLAG and human STAT1 aa1-508 joined to human STAT…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    134723-plasmid-map-sequence-id-272780

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Showing: 1441 - 1460 of 1837 results