Addgene

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CycA-Venus-Flag (1305)

Plasmid

#39853

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

Insert

Cyclin A (CCNA2 Human)

Use

Tags

Flag and Venus

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pBS-IT6

Plasmid

#66870

Purpose

Inntag 6: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 6

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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AiP13278: pAAV-AiE0779m_3xC2-minBG-ChR2(CRC)-EYFP-WPRE3-BGHpA (Alias: CN3278)

Plasmid

#214843

Purpose

Expression of ChR2(L132C, H134R, T159C)-EYFP in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

ChR2(L132C, H134R, T159C)-EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJPF0190

Plasmid

#130268

Purpose

Piggybac vector for the expression of mRuby2-fused human PRKACA

Depositor

Hana El-Samad

Article

Fonseca et al bioRxiv 2020, 747030

Insert

PRKACA (PRKACA Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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AiP15143 - pAAV-AiE0452h-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN5143)

Plasmid

#224193

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNs

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

mScarlet-10aa-H2B

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13445 - pAAV-AiE0873m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3445)

Plasmid

#191725

Purpose

AI plasmid ALIAS: AiP13445. Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Use

Tags

luciferase

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only
EGFP-supervillin

Plasmid

#13040

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-UL32

Plasmid

#162658

Purpose

Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSTK120-mCMV-TK

Plasmid

#22054

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

mCMV-TK-pA expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pBS-IT5

Plasmid

#66869

Purpose

Inntag 5: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 5

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only
DL034:cFos::HaloTag-iPAK4

Plasmid

#177884

Purpose

HaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

iPAK4

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

cFos

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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VZV ORF47

Plasmid

#26698

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

ORF47 (ORF47 Varicella Zoster Virus (HHV-3))

Use

Tags

HA

Expression

Mammalian

Mutation

A 3B3 epitope tag (DQRQYGDVFKGD) from the VZV gB …

Promoter

Available since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only
pTK105

Plasmid

#59562

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PEST

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn EYFP-PEST

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_zyxin

Plasmid

#83034

Purpose

Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_zyxin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

Plasmid

#211491

Purpose

conditional expression of GCaMP8s under artificial promoter Adamts2

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

Plasmid

#211493

Purpose

conditional expression of GCaMP8s under artificial promoter Baz1a

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSTAP-seq_human_spike-in

Plasmid

#125152

Purpose

Empty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Type

Empty backbone

Use

Stap-seq screening vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTK298

Plasmid

#59567

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBB14

Plasmid

#18657

Depositor

Lynn Enquist

Article

Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

Insert

Us9-EGFP gene fusion

Use

Tags

EGFP

Expression

Mammalian

Mutation

First 106 amino acids of Us9 are intact. The stop…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge