We narrowed to 162,094 results for: addgene

1. TAL2065

   Plasmid

   #39435

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-39-Right
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

2. TAL2064

   Plasmid

   #39434

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-39-Left
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCJ1021

   Plasmid

   #228761

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 gRNAs (Ift88 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-HypaCas9

   Plasmid

   #190604

   Purpose

   PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNM3755

   Plasmid

   #154116

   Purpose

   tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   tetO 7X::∆pes-10::GFP-C1::act-4 3'
   

   Use

   Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DCAF1:PBC020-D05:C246337

   Plasmid

   #183974

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   DCAF1:PBC020-D05:C246337 (DCAF1 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV and MHHHHHHSSGRENLYFQG

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pNM3823

   Plasmid

   #154123

   Purpose

   mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   mec-4p::tetR-L-QFAD::act-4 '3
   

   Use

   Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LRG2.1-CyOFP1

   Plasmid

   #124771

   Purpose

   3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBABE-EGFP

   Plasmid

   #36999

   Depositor

   Debu Chakravarti

   Article

   Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Halo-TMD

    Plasmid

    #195347

    Purpose

    Fluorescent labeling for cell membranes

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6CC8 (MBD3)

    Plasmid

    #119959

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD3 (MBD3 Human)
    

    Use

    Tags

    GSTtag, 6xHis and Thrombin

    Expression

    Bacterial

    Mutation

    MBD domain (contains residues 1-71)

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. MXS_CreERT2

    Plasmid

    #62427

    Purpose

    MXS_chaining vector with CreERT2

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Tamoxifen inducible Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti SpBsmBI sgRNA Puro

    Plasmid

    #62207

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNM3774

    Plasmid

    #154117

    Purpose

    tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆mec-7p::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pKL09-GCaMP6f-mScarlet bb118

    Plasmid

    #158979

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-mScarlet fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6BY9 (EHMT1)

    Plasmid

    #119956

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    EHMT1 (EHMT1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBRα

    Plasmid

    #53731

    Purpose

    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    αNTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. LRG2.1-mOrange

    Plasmid

    #124772

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pN3-3xFlag-Control

    Plasmid

    #107717

    Purpose

    Control expression plasmid for 3xFLAG-tagged pN3-contructs

    Depositor

    Guntram Suske

    Article

    Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

    Type

    Empty backbone

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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