Skip to main content
Addgene
Showing: 3651 - 3700 of 18314 results

  2. pGL4.10/RSV_XS3B-miniXon_Luciferase

    Plasmid
    #217302
    Purpose
    Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by L
    Depositor
    Beverly L. Davidson
    Insert
    XS3B-miniXon
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    217302-plasmid-map-sequence-id-436485

    Enlarge

  3. AAV-PR rep/cap

    Plasmid
    #197565
    Purpose
    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery
    Depositor
    Casey Maguire
    Article
    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089
    Inserts
    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    Use
    AAV
    Tags
    Expression
    Mutation
    contains 7mer insert PRPPSTH between amino acids …
    Promoter
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197565-plasmid-map-sequence-id-388046

    Enlarge

  8. pMXS-GshF-blasticidin

    Plasmid
    #210313
    Purpose
    Expresses humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Kivanc Birsoy
    Article
    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide
    Expression
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    210313-plasmid-map-sequence-id-418847

    Enlarge

  9. MAVS-Mito

    Plasmid
    #44556
    Purpose
    Retroviral vector for expression of a chimeric MAVS allele targeted to mitochondria in mammalian cells
    Depositor
    Jonathan Kagan
    Article
    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20
    Insert
    MAVS-Fis1 chimeric protein (MAVS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    contains aa1-500 of MAVS and aa127-152 of Fis1
    Promoter
    Available since
    Aug. 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. MAVS-Pex

    Plasmid
    #44557
    Purpose
    Retroviral vector for expression of a chimeric MAVS allele targeted to peroxisomes in mammalian cells
    Depositor
    Jonathan Kagan
    Article
    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20
    Insert
    MAVS-Pex13 chimeric protein (MAVS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    contains aa1-500 of MAVS and aa136-233 of Pex13
    Promoter
    Available since
    Aug. 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pCEP4-M2L

    Plasmid
    #20926
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Inserts
    MYC (MYC Human)
    LIN28 (LIN28A Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    CMV and IRES
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20926-plasmid-map-sequence-id-9762

    Enlarge

  23. pB-rtTA

    Plasmid
    #126034
    Purpose
    PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.
    Depositor
    Mauro Calabrese
    Article
    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do
    Insert
    rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hUbiC
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    126034-plasmid-map-sequence-id-245792

    Enlarge

  24. PL-SIN-EOS-C(3+)-EiP

    Plasmid
    #21313
    Purpose
    Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance
    Depositor
    James Ellis
    Article
    Hotta et al Nat Methods. 2009 May . 6(5):370-6.
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EOS-C(3+) promoter region
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    21313-plasmid-map-sequence-id-9854

    Enlarge

  25. PL-SIN-EOS-S(4+)-EiP

    Plasmid
    #21314
    Purpose
    Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance
    Depositor
    James Ellis
    Article
    Hotta et al Nat Methods. 2009 May . 6(5):370-6.
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EOS-S(4+) promoter region
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    21314-plasmid-map-sequence-id-305770

    Enlarge

  29. pAAV.cTNT.iCre

    Plasmid
    #69916
    Purpose
    Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.
    Depositor
    William Pu
    Article
    Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:
    Insert
    improved cre (iCre) recombinase and tdTomato
    Use
    AAV
    Tags
    The iCre coding frame and tdTomato coding frame i…
    Expression
    Mutation
    Promoter
    Chicken cardiac troponin T
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    69916-plasmid-map-sequence-id-124095

    Enlarge

  34. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    90086-plasmid-map-sequence-id-182687

    Enlarge

  35. FUW-tetO-EGFP

    Plasmid
    #84041
    Purpose
    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
    Depositor
    Stefano Piccolo
    Article
    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    84041-plasmid-map-sequence-id-164306

    Enlarge

Showing: 3651 - 3700 of 18314 results