We narrowed to 3,025 results for: GRI

1. pMV_AA090

   Plasmid

   #216099

   Purpose

   Knockout, EF1a-driven Cas12a (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas12a [EnAs]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-puro-sh-mFAK C

   Plasmid

   #37015

   Depositor

   Bob Weinberg

   Article

   Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

   Insert

   sh-mFAK C (Ptk2 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 31, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 6A CARD8 CARD-FLAG WT

   Plasmid

   #164020

   Purpose

   Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 CARD8

   Plasmid

   #169962

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA009

   Plasmid

   #216037

   Purpose

   Fragmid fragment: (C' terminus) Nucleoplasmin NLS

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   NpNLS_v1.1; 3xHA_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. rTetR-GFP-VP16

   Plasmid

   #183917

   Purpose

   VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   rTetR-GFP-VP16 (UL48 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA345

   Plasmid

   #216031

   Purpose

   Fragmid fragment: (Cas protein) Cas13d

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas13d_v1.1 [Dj]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_834

   Plasmid

   #216087

   Purpose

   Cas9 [Sp] knockout targeting CD59, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD59 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIC292

   Plasmid

   #51405

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska2 IMAGE 4685972 (SKA2 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJM160

    Plasmid

    #134169

    Purpose

    Bacterial expression of RB3 stathmin-like domain (SLD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    RB3-SLD (STMN4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues 49-189

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-puro-sh-mFAK B

    Plasmid

    #37018

    Depositor

    Bob Weinberg

    Article

    Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

    Insert

    sh-mFAK B (Ptk2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pEGFP-N3/hArf3(WT)-EGFP

    Plasmid

    #79418

    Purpose

    Expresses C-terminally EGFP-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (ARF3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HS-OS-Plk4-mKate2

    Plasmid

    #105944

    Purpose

    heat shock inducible transgenesis, non phoshorylable version of Plk4

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Plk4 (Plk4 Mouse)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    1002 to 3704 of Plk4/ deletion of S282-S305.

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. isoform PKC lambda (JCE590)

    Plasmid

    #89775

    Purpose

    yeast expression of mouse PKC lambda with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Article

    Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

    Insert

    PKC lambda (Prkci Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N3/hArf1(WT)-EGFP

    Plasmid

    #79413

    Purpose

    Expresses C-terminally EGFP-tagged Arf1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

    Plasmid

    #128609

    Purpose

    Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    uORF1-36xMashTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)

    Plasmid

    #114212

    Purpose

    Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-puro-mIR-31 sponge

    Plasmid

    #22694

    Purpose

    Stable suppression of miR-31 function

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Tags

    *

    Expression

    Mammalian

    Mutation

    miR-31 sponge with 7x tandem binding sites to the…

    Promoter

    Available since

    March 11, 2010

    Availability

    Academic Institutions and Nonprofits only

19. ALFY-FYVE 1G

    Plasmid

    #197045

    Purpose

    Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

    Insert

    ALFY (WDFY3 Human)
    

    Use

    Tags

    6xHis-GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3/mArf3(T31N)-HA

    Plasmid

    #79416

    Purpose

    Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (Arf3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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