We narrowed to 2,088 results for: K-Ras protein

1. pEPQD0KN0244

   Plasmid

   #162282

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEPQD0KN0024

   Plasmid

   #162281

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)Gαi3-91-NLuc

   Plasmid

   #207150

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI3 (GNAI3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)K

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCellFree_G06_ZIKV E

   Plasmid

   #145351

   Purpose

   Cell-free expression vector for Zika virus Envelope protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV Envelope
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmCellFree_KA1_ZIKVC

   Plasmid

   #145339

   Purpose

   Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV Capsid
   

   Use

   Tags

   8xHis and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   April 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCellFree_G06_ZIKV NS5

   Plasmid

   #145358

   Purpose

   Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS5
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCellFree_G06_ZIKV NS3

   Plasmid

   #145355

   Purpose

   Cell-free expression vector for Zika virus NS3 protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS3
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCellFree_G06_ZIKVC

   Plasmid

   #145349

   Purpose

   Cell-free expression vector for Zika virus Capsid protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV Capsid
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCellFree_G06_ZIKV NS1

   Plasmid

   #145352

   Purpose

   Cell-free expression vector for Zika virus NS1 protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS1
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCellFree_G06_ZIKV NS4B

    Plasmid

    #145357

    Purpose

    Cell-free expression vector for Zika virus NS4B protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS4B
    

    Use

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCellFree_G06_ZIKV NS2A

    Plasmid

    #145353

    Purpose

    Cell-free expression vector for Zika virus NS2A protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2A
    

    Use

    Tags

    mCherry and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCellFree_G06_ZIKV PrM

    Plasmid

    #145350

    Purpose

    Cell-free expression vector for Zika virus prM protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV PrM
    

    Use

    Tags

    mCherry and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCellFree_G06_ZIKV NS4A

    Plasmid

    #145356

    Purpose

    Cell-free expression vector for Zika virus NS4A protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS4A
    

    Use

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCellFree_G06_ZIKV NS2B

    Plasmid

    #145354

    Purpose

    Cell-free expression vector for Zika virus NS2B protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2B
    

    Use

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4-UPRE-luc2P-Hygro

    Plasmid

    #101788

    Purpose

    Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xUPRE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28_Strep-Psc-pScaC

    Plasmid

    #228834

    Purpose

    E. coli expression of the ScaC passenger domain

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 33-223

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28_pScaC(50-159)

    Plasmid

    #228836

    Purpose

    E. coli expression of a truncated ScaC passenger domain (aa 50-159)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 50-159

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCerulean3-Rab4a-7

    Plasmid

    #55443

    Purpose

    Localization: Ras GTPase, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET22b_pScaC-SNAP_6xHis

    Plasmid

    #228835

    Purpose

    E. coli expression of the SNAP-tagged ScaC passenger domain

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    SNAPtag-TEV-6xHis

    Expression

    Bacterial

    Mutation

    aa 33-223

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSM.wrmScarlet1-10::SL2::GFP1-10

    Plasmid

    #234318

    Purpose

    For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

    Insert

    wrmScarlet1-10::SL2::GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge