Addgene

We narrowed to 4,775 results for: Mos

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pKIKOlacZKm

Plasmid

#46767

Purpose

E.coli genomic integration at lacZ

Depositor

Claudia Vickers, Lars Nielsen

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pTmpGm

Plasmid

#176229

Purpose

A template plasmid for amplification of the cI-hok-aacC4 cassette

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

cI of phage lambda, hok of the R1 plasmid, aacC4

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MCP-YFP

Plasmid

#101160

Purpose

to express MCP-YFP fusion protein

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MCP-YFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pTcI42-Wasabi

Plasmid

#86118

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI42
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

A67T, K6N, S33T, Y61H, L119P, F122C

Promoter

pR/pL and pR/pL, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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H2B-mNeonGreen-IRESpuro2

Plasmid

#183745

Purpose

Expresses H2B-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A22

Plasmid

#166210

Purpose

Gateway entry vector for N. furzeri SLC25A22

Depositor

Daniel Jarosz

Article

Yiwen R. Chen et al bioRxiv

Insert

SLC25A22

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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mCD8-GFP in pUAST

Plasmid

#17746

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

mouse CD8 (Cd8a Mouse)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pKIKOarsBKm

Plasmid

#46766

Purpose

E.coli genomic integration at arsB

Depositor

Claudia Vickers, Lars Nielsen

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Use

Tags

Expression

Bacterial

Mutation

Promoter

no promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only
pTlpA-Wasabi-NF

Plasmid

#86100

Purpose

Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A and S71T, G73A

Promoter

TlpA and TlpA, LacI

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-TID-HA3-KanMX6

Plasmid

#227980

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBTSG

Plasmid

#159420

Purpose

Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Use

Tags

polyhistidine (poly-His)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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MX2_pCSdest

Plasmid

#53942

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MX2 (MX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSKI074

Plasmid

#11571

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Tags

Expression

Plant

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pFA6a-TID-HA3-HIS3MX6

Plasmid

#227981

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKM446 (Flag-DAS+4))

Plasmid

#108321

Purpose

Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Flag-DAS+4

Use

Orbit integrating plasmid

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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