We narrowed to 9,242 results for: ef1

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pAAV-Ef1a-fDIO-mRuby3-2A-dCre

Plasmid

#203845

Purpose

Flp-dependent enzymatically inactive Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mRuby3-2A

Mutation

Enzymatically inactive Cre

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

Plasmid

#202617

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

FRT-JEDI-1P-Kv

Use

AAV; Flp/frt

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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Pb459-mU6-sgRNA-EF1a-PuroR

Plasmid

#195507

Purpose

piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Puromycin

Use

CRISPR; Piggybac

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_r5-HT1.0

Plasmid

#208725

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR[EF1α-copGFP]

Plasmid

#218327

Purpose

Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA

Plasmid

#37086

Purpose

Expresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-YFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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C413-E15: attB4-EF1p>-attB1r

Plasmid

#162920

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-full-length-CHMP3-HA

Plasmid

#154176

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-tetR-T2A-iRFP

Plasmid

#176028

Purpose

construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

tetR
iRFP

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

Plasmid

#185060

Purpose

Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445

Depositor

Bobby Koeleman

Insert

U6:pegRNA:scaffold:PBS+RT template

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-GFP-miRE_shRen-Blast

Plasmid

#135682

Purpose

Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

Depositor

Florian Karreth

Insert

shRenilla

Use

Lentiviral

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-EF1a-mTagBFP2

Plasmid

#160507

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Lv EF1a HP1cs-Frb2x PGK Puro

Plasmid

#102809

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

HP1cs-Frb2x

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

Plasmid

#208724

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mCherry-WPRE

Plasmid

#202620

Purpose

Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pTYF-EF1a-DLDH-IRES-EGFP

Plasmid

#178310

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-AcGFP-P2A-hBRD4S

Plasmid

#197882

Purpose

study roles of human BET proteins in human pluripotency reprogramming

Depositor

Kejin Hu

Article

Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

Plasmid

#69587

Purpose

Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

p53 L344P mutation that prevents dimerization and…

Promoter

EF1alpha

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-BLST-Cas9-VPR

Plasmid

#115147

Purpose

Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-Cas9-VPR

Use

CRISPR

Tags

SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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