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pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160C

Plasmid

#46

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

expresses aa580-1330

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
NmeCas9_3XNLS_pMCSG7

Plasmid

#120078

Purpose

Bacterial expression of wtNmeCas9 with 3X NLS

Depositor

Erik Sontheimer

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

NmeCas9

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pOAD

Plasmid

#196825

Purpose

Yeast two-hybrid ; Gal4 activation domain

Depositor

Stan Fields

Article

Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

Type

Empty backbone

Tags

Gal4 Activation domain

Expression

Yeast

Promoter

ADH

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-H-Ras_wt

Plasmid

#39509

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS (HRAS Human)

Use

Eukaryotic

Tags

HA

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
AAV-tLuc

Plasmid

#107552

Purpose

tLuc in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Luciferase

Use

AAV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_dNmeCas9

Plasmid

#120077

Purpose

dNmeCas9 with no sgRNA cassette lenti construct

Depositor

Erik Sontheimer

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

dNmeCas9

Use

Lentiviral

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160(1-200)

Plasmid

#43

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(400-580)

Plasmid

#45

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

expresses only aa400-580

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(200-400)

Plasmid

#44

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

Expresses only aa200-400

Available Since

July 21, 2005

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12

Plasmid

#39510

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Eukaryotic

Mutation

G12V

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 ATG wt

Plasmid

#52034

Purpose

encodes wt HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

starting ATG deleted

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pUDR211

Plasmid

#113870

Purpose

expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA3-HXT8 / sgRNA4-HXT14

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00167

Plasmid

#34320

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12399 (Mad Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-S35

Plasmid

#39511

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and T35S

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-G37

Plasmid

#39512

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and E37G

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-C40

Plasmid

#39515

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 C40 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and Y40C

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 ATG m7

Plasmid

#52035

Purpose

encodes mutant HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

all seven let-7 sites in the 3′ UTR mutated (‘m7’)

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pTCas9_scr

Plasmid

#207566

Purpose

ThermoCas9-mediated cleavage of genomic DNA in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGCas9_scr

Plasmid

#207567

Purpose

GeoCas9-mediated cleavage of genomic DNA in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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