We narrowed to 2,684 results for: Kl

1. pcDNA5-FRT/TO-N-mEGFP-PARP6

   Plasmid

   #178005

   Purpose

   Expresses N-terminally mEGFP-tagged PARP6

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP6 (PARP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-N-mEGFP-PARP11

   Plasmid

   #177998

   Purpose

   Expresses N-terminally mEGFP-tagged PARP11

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP11 (PARP11 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

   Plasmid

   #113436

   Purpose

   Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   type II-C anti-CRISPR
   

   Use

   CRISPR

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-puro FLAG wildtype, catalytic active lipin 1

   Plasmid

   #32010

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   lipin1 (Lpin1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCsy_complex

   Plasmid

   #89232

   Purpose

   Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

   Depositor

   Blake Wiedenheft

   Article

   Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

   Inserts

   Csy1
   Csy2
   Csy3
   Csy4
   

   Use

   Tags

   6xHis-TEV protease

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLO 64.1

   Plasmid

   #123120

   Purpose

   E. coli expression vector for active deltaproteobacteria CasX

   Depositor

   Jennifer Doudna, Benjamin Oakes

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   CasX
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   NA

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Danio alpha A-crystallin 1kb promoter/AcGFP

   Plasmid

   #98096

   Purpose

   aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
   

   Use

   Gfp expressing

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Danio alpha A-crystallin 1 kb fragment (-1028/-1)

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

   Plasmid

   #136514

   Purpose

   Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

   Depositor

   Erik Sontheimer

   Article

   Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

   Insert

   Codon-optimized AcrIIA5
   

   Use

   Lentiviral

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACEBac1-PARP1-Flag-His-7xKQ

   Plasmid

   #111573

   Purpose

   Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   lysine to glutamine mutation at the following ami…

   Promoter

   polyhedrin promoter

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBLO ddd64.1

    Plasmid

    #123121

    Purpose

    E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Mutated amino acids in catalytic triad to alanine…

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. tdKatushka2-Fibrillarin-7

    Plasmid

    #56041

    Purpose

    Localization: Nucleoli, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pblic-neo TRX1

    Plasmid

    #110919

    Purpose

    To overexpress Thioredoxin-1

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNAintron-CHIKV-Structural polyprotein

    Plasmid

    #215698

    Purpose

    Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV structural polyprotein Capsid-E3-E2-6K-E1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pMH-SFB-DYRK1B

    Plasmid

    #101771

    Purpose

    Mammalian expression of DYRK1B fusion protein

    Depositor

    Michael Huen

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

    Insert

    DYRK1B / MIRK (DYRK1B Human)
    

    Use

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

15. MIGR1-cyclin E-AA

    Plasmid

    #47498

    Purpose

    MSCV-based vector encodes human cyclin E mutated within N- and C-terminal Cdc4 phosphodegrons

    Depositor

    Alex Minella

    Article

    Xu et al Oncogene. 2013 Jul 22. doi: 10.1038/onc.

    Insert

    cyclin E1 (CCNE1 Human)
    

    Use

    Retroviral; Mscv-ires-gfp

    Tags

    Expression

    Mammalian

    Mutation

    threonines 62 and 380 to alanine (T62A T380A)

    Promoter

    LTR

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pET28-HADH2

    Plasmid

    #67863

    Purpose

    bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    HADH2 (HSD17B10 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EFS-FlpO

    Plasmid

    #191509

    Purpose

    Lentiviral Flp Recombinase driven by the EFS promoter

    Depositor

    Eric Snyder

    Article

    Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

    Insert

    flp recombinase (flp )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/EFS promoter inserted

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. integrin alpha9 pcDNA1 neo

    Plasmid

    #13585

    Depositor

    Dean Sheppard

    Article

    Yokosaki et al J Biol Chem. 1994 Oct 28. 269(43):26691-

    Insert

    integrin alpha 9 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

    Plasmid

    #110300

    Purpose

    Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBG-bZIPa

    Plasmid

    #12436

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    bZIP of C/EBPa (CEBPA Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    pEBG vector is from Bruce Mayer, may be different…

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only