We narrowed to 5,913 results for: pcDNA 3.1

1. pcDNA3.1 human cofilin S3A

   Plasmid

   #50854

   Purpose

   expresses constituvely active cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3A (CFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   constitutively active, S3A

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pcDNA3.1-Mito-AcGFP-MTn

   Plasmid

   #154350

   Purpose

   Mammalian expression of MTn

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   MTn
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-Ost4-GFP-MTn

   Plasmid

   #154351

   Purpose

   Mammalian expression of MTn

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   MTn
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

   Plasmid

   #98041

   Purpose

   Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Insert

   Sa-dCas9-NLS-3xFLAG
   

   Use

   CRISPR

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   D10A + H557A

   Promoter

   CMV

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Rab8-T22N(Flag) in pcDNA3.1neo

   Plasmid

   #46784

   Purpose

   expression of T22N Rab8a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB8A T22N (RAB8A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T22N dominant negative

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1 CIM5 (Rpt-1) GFP

   Plasmid

   #16074

   Depositor

   Erin Schuman

   Article

   Bingol et al Nature. 2006 Jun 29. 441(7097):1144-8.

   Insert

   CIM5 (RPT1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA 3.1-nsp7-FLAG

   Plasmid

   #201027

   Purpose

   Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)-ExRai-AKAR-NES

   Plasmid

   #118407

   Purpose

   Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-NES
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-CoV2-Omicron BA.1 spike

   Plasmid

   #194602

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

   Depositor

   Ben Murrell

   Article

   Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

   Insert

   CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

    Plasmid

    #181859

    Purpose

    Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    cp-PKN-mRuby2-GFP(1-0)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1/NDUFV1/myc-His

    Plasmid

    #127490

    Purpose

    Expresses myc-tagged NDUFV1 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV1 (NDUFV1 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-2xFLAG-2xSTREP-CDK2

    Plasmid

    #172616

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-ERBB4 (kinase dead)

    Plasmid

    #29533

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed lysine 751 to methionine

    Promoter

    Available since

    July 20, 2011

    Availability

    Academic Institutions and Nonprofits only

14. mTiam1-mcherry-sspB in pcDNA3.1

    Plasmid

    #85221

    Purpose

    Role of Rac selective GEF domain from Tiam1 in immune cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Inserts

    mTiam1 (1030-1406) (Tiam1 Mouse)
    mcherry
    sspB
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA 3.1 KBTBD4 R313PRR

    Plasmid

    #184629

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R313PRR expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R313PRR (KBTBD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R313PRR

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cry2 mCherry GRK2 ct in pcDNA3.1

    Plasmid

    #64212

    Purpose

    mCherry fused between Cry2 and GRK2ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK2 ct (GRK2 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and cmv

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

17. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

    Plasmid

    #52502

    Purpose

    expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    deletion of Fbox (deleted aa 71-116)

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)-mCherry-p115(S240A)

    Plasmid

    #181997

    Purpose

    Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mCherry-p115(S240A) (ARHGEF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Serine 240 changed to alanine in p115.

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-CMV-PdCO-EGFP

    Plasmid

    #198512

    Purpose

    Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-TAT-2-130-FLAG

    Plasmid

    #35984

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV2 130 amino acids

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge