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Fucci(CA) hCdt1-iRFP hGeminin-iRFP

Plasmid

#190182

Purpose

Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
iRFP
hGeminin(1/110)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pNCS-mGold2t

Plasmid

#231766

Purpose

Expression of mGold2t in bacterial cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t

Expression

Bacterial

Mutation

mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Synthetic promoter

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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Bcr/Abl P210-pLEF

Plasmid

#38158

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

BCR/ABL P210 (ABL1, BCR Human)

Tags

GST

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold2t

Plasmid

#231764

Purpose

Expression of mGold2t in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t

Expression

Mammalian

Mutation

mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Actin promoter with CMV enhancer

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-MYO5C

Plasmid

#135404

Purpose

Expresses GFP-tagged human MYO5C in mammalian cells.

Depositor

Richard Cheney

Article

Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

Insert

MYO5C (MYO5C Human)

Tags

EGFP

Expression

Mammalian

Mutation

Relative to the GenBank MYO5C cDNA sequence repor…

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Delta Spike

Plasmid

#185593

Purpose

Expresses codon-optimized full length SARS-CoV-2 Delta Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Delta Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19R, Δ156-157,…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

Plasmid

#123615

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A/K34A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A/K34A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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TRCN0000338462

Plasmid

#78158

Purpose

shXPO1 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

XPO1 (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pRSITEP-puro-shWRN1

Plasmid

#125783

Purpose

DOX-inducible expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shRFP

Plasmid

#125778

Purpose

constitutive expression of a short-hairpin RNA targeting RFP

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRFP

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN2

Plasmid

#125785

Purpose

DOX-inducible expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN1-C911

Plasmid

#125784

Purpose

a seed-matched control for pRSITEP-puro-shWRN1

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1-C911 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN2-C911

Plasmid

#125786

Purpose

a seed-matched control for pRSITEP-puro-shWRN2

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN1

Plasmid

#125781

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1 (WRN Human)

Use

RNAi

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFGC-I2Cas9

Plasmid

#173158

Purpose

Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

Depositor

Piotr Ziolkowski

Article

Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

Inserts

hSpCas9
pNAP:dsRED:tNOS
pMAS:BAR:tMAS
ICU2 upstream regulatory region

Tags

3xFLAG-NLS and NLS

Expression

Plant

Mutation

Contains a D169N mutation with no visible effect …

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shWRN2

Plasmid

#125782

Purpose

constitutive expression of a short-hairpin RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN2 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

Plasmid

#113158

Purpose

An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV, CRISPR, and Cre/Lox

Expression

Mammalian

Promoter

mU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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BE3(E63Q)-P2A-EGFP (pRZ189)

Plasmid

#123613

Purpose

CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(E63Q)-P2A-EGFP

Expression

Mammalian

Mutation

E63Q in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN1

Plasmid

#125771

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN1 (WRN Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shPSMD2

Plasmid

#125779

Purpose

constitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shPSMD2 (PSMD2 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN2

Plasmid

#125772

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN2 (WRN Human)

Use

CRISPR

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgCh2-2

Plasmid

#125775

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgWRN3

Plasmid

#125773

Purpose

constitutive expression of a guide RNA targeting human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN3 (WRN Human)

Use

CRISPR

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-FLAG-MKK6

Plasmid

#21582

Depositor

John Kyriakis

Article

Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

Insert

MKK6 (MAP2K6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

Plasmid

#244178

Purpose

Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Human, Synthetic)

Use

Synthetic Biology

Tags

TNFR1 signal peptide - 3xFLAG

Expression

Mammalian

Mutation

CTEVp_190K

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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L4341 TNFR1 NTEVp chain (deleted MMP site) in PolyTX-mNeonGreen

Plasmid

#244176

Purpose

Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-NTEVp(75S)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

MESA NTEVp chain with human TNFR1 SS and ECD (no MMP site), murine CD28 TMD, and NTEVp 75S mutant (TNFRSF1A Human, Synthetic)

Use

Synthetic Biology

Tags

TNFR1 signal peptide - 3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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rTTA-gRNA-AAV

Plasmid

#213036

Purpose

This vector contains rTTA expressed under CMV promoter and gRNA expressed under U6 promoter

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

rTTA-T2A-mCherry

Use

AAV and CRISPR

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pJL-mGold2s

Plasmid

#231761

Purpose

Expression of mGold2s in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Yeast

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

pTDH(GAP promoter)

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pJL-mGold2t

Plasmid

#231762

Purpose

Expression of mGold2t in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t

Expression

Yeast

Mutation

mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

pTDH(GAP promoter)

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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