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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJAID6JY

Plasmid

#165049

Purpose

Integrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID8Z105

Plasmid

#165057

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID9L105

Plasmid

#165055

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID9L

Plasmid

#165052

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID5Z

Plasmid

#165047

Purpose

Integrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

OsTIR1 is codon-optimised for expression in S. ce…

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Bth

Plasmid

#129686

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty Backbone

Use

CRISPR

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1226

Plasmid

#138593

Purpose

Expresses NatL2-R156A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Arginine 156 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1222

Plasmid

#138592

Purpose

Expresses NatL2-K418A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Lysine 418 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Bh

Plasmid

#136378

Purpose

Gateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag

Plasmid

#129255

Purpose

Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST

Plasmid

#129264

Purpose

Express soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag-ST

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST

Plasmid

#129263

Purpose

Express soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag-ST

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

Plasmid

#129260

Purpose

Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag-ST

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Mac_Q139D_D150N_E258V-HaloTag

Plasmid

#129259

Purpose

Express Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag

Plasmid

#129258

Purpose

Express QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/68

Plasmid

#113921

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/66

Plasmid

#113920

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/66

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pT7T3D-PacI-Tmeff2

Plasmid

#99728

Purpose

to make in situ RNA probes for Tmeff2

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Use

In situ probe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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POT5-TDH3p-ycrtYB-ADH1t

Plasmid

#65358

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtYB-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-TDH3p-ycrtE-ADH1t

Plasmid

#65352

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtE-ADH1t

Use

Unspecified

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_WDR91:1-747

Plasmid

#210996

Purpose

Mammalian expression of full-length human WDR91. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_PREB:1-417

Plasmid

#211131

Purpose

Mammalian expression of full-length human PREB. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PREB:M1-L417 (PREB Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR77:1-342

Plasmid

#211169

Purpose

Mammalian expression of full-length human WDR77. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR77:M1-E342 (WDR77 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_NUP214:1-2090

Plasmid

#211294

Purpose

Mammalian expression of full-length human NUP214. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NUP214:M1-S2090 (NUP214 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

P574S natural variant (VAR_045691). S1872N annot…

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_STRN:1-731

Plasmid

#211230

Purpose

Mammalian expression of full-length human STRN isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

STRN:M1-V731 (STRN Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_WDR62:1-1518

Plasmid

#211257

Purpose

Mammalian expression of full-length human WDR62 isoform 4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR62:M1-H1518 (WDR62 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 4. L850S natural VAR_031299. Q1305L n…

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_PPP2R2A:1-447

Plasmid

#211321

Purpose

Mammalian expression of full-length human PPP2R2A. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PPP2R2A:M1-N447 (PPP2R2A Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_PALB2:1-1186

Plasmid

#211160

Purpose

Mammalian expression of full-length human PALB2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PALB2:M1-S1186 (PALB2 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_RACK1:1-317

Plasmid

#211110

Purpose

Mammalian expression of full-length human RACK1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RACK1:M1-R317 (RACK1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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