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Showing: 2221 - 2250 of 2924 results
  1. pJW1927

    Plasmid
    #163093
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Insert
    mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

    Plasmid
    #229810
    Purpose
    A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Insert
    loxp-AmCyan-stop-lox
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

    Plasmid
    #229816
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.
    Depositor
    Insert
    loxp-EGFP-stop-lox
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid
    #229841
    Purpose
    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre
    Depositor
    Insert
    loxp-H2B-mCerulean-2xStop-loxP
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. Donor-CD55-NeoR

    Plasmid
    #153551
    Purpose
    Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Insert
    mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Expression
    Mutation
    a 1-bp mutation in exon 4
    Promoter
    No promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. Donor-PIGA-NeoR

    Plasmid
    #153549
    Purpose
    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Expression
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 2221 - 2250 of 2924 results