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pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130995

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

hSyn

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x2

Plasmid

#63596

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D1361Y

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100851

Purpose

AAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-ATF3

Plasmid

#26115

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF3 (ATF3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pBV-Luc wt MBS1-4

Plasmid

#16564

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Rheb

Plasmid

#19996

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 (RHEB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24

Plasmid

#28138

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

Plasmid

#75470

Purpose

Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Reversed ChR2(H134R)-mCherry

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GFP-fGFP

Plasmid

#135631

Purpose

AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV1 and AAV9

Insert

GFP-fGFP

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-KD LKB1

Plasmid

#8593

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K78I: lysine 78 mutated to isoleucine; kinase dead

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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Myc tag WT PTB

Plasmid

#23024

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pLNCX2-mCherry-CHMP4B

Plasmid

#116923

Purpose

expresses mCherry-CHMP4B in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

CHMP4B (CHMP4B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-NOS2Promoter-Luciferase

Plasmid

#19296

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

Insert

NOS2 Promoter (Nos2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV.GfaABC1D.PI.Lck-GFP.SV40

Plasmid

#105598

Purpose

AAV expression of membrane bound EGFP from GfaABC1D promoter

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has Service

AAV5

Insert

Lck-EGFP

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMB80 (R26-CreER)

Plasmid

#12168

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Rosa26 CreER^T2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-iLID-CAAX (from KRas4B)

Plasmid

#60411

Purpose

Mammalian Expression of Venus-iLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET302-6His-dCas9-Halo

Plasmid

#72269

Purpose

Encodes a Halo-labeled fusion of nuclease deficient Cas9

Depositor

Timothée Lionnet

Article

Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

Insert

His6-dCas9-Halo

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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