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pH2B-mPlum/Gag

Plasmid

#102255

Purpose

co-expression of Gag and H2B Plum in mammalian cells

Depositor

Suliana Manley

Article

Gunzenhauser et al PLoS One. 2014 Dec 10;9(12):e115095. doi

Inserts

Gag
H2B Plum

Expression

Mammalian

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGLOW30

Plasmid

#172697

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-2

Available Since

Sept. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX

Plasmid

#202718

Purpose

Mammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Pink Flamindo
paGFP-bPAC-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTETDuet_M42FG121VDHFR_R166QTS

Plasmid

#191298

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F/G121V DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-TOMM20

Plasmid

#227307

Purpose

Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE5max-SF3B1-K700E

Plasmid

#200317

Purpose

Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

Depositor

Lili Wang

Article

Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

Inserts

K700E-ng1+pg2
PEmax-2A-MLH1dn

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

Plasmid

#104097

Purpose

Expresses TREAT-PK co-localization control in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCXLE-hK + E2-Crimson

Plasmid

#74946

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Klf4 (KLF4 Human)

Expression

Mammalian

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203228

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Membrane-localized optopatch for neuronal dendrites

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

Plasmid

#176280

Purpose

Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mNaChBac-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-ZHER-mgSrtA

Plasmid

#125793

Purpose

for co-expression of ZHER (a HER2 binding affibody) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of HER2 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

ZHER-mgSrtA

Expression

Mammalian

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Plasmid

#182063

Purpose

Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

Depositor

Adam Cohen

Article

Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

Insert

QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Use

Lentiviral

Promoter

CAG

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(rInsp)-Ins-GLuc

Plasmid

#89928

Purpose

Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

Depositor

Melanie Cobb

Article

Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

Insert

human insulin-Gaussia-Luciferase (INS Synthetic, Human)

Expression

Mammalian

Mutation

DNA sequence for Gaussia luciferase was humanized…

Promoter

rat Insulin II promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-trAtCCD7-OsD27(pYL726 )

Plasmid

#178285

Purpose

Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

OsD27
trAtCCD7

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP

Plasmid

#176282

Purpose

Viral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

TeNT-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

Plasmid

#125796

Purpose

for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L (lose of function mutant)-mgSrtA

Expression

Mammalian

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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anti-FIX(a) Emi HC

Plasmid

#113664

Purpose

For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.

Depositor

Robert Peters

Article

Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1

Insert

Emicizumab anti-FIX/FIXa heavy chain

Expression

Mammalian

Promoter

CMV Promoter

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-p300-P2A-CASANOVA

Plasmid

#113129

Purpose

Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

dCas9-p300-P2A-CASANOVA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

Plasmid

#202719

Purpose

Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Myr-Pink Flamindo
EGFP-bPAC-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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