We narrowed to 2,990 results for: SRS

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pECE-M2-AMPKalpha2 wt

Plasmid

#31652

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE GFP-Ago2 S387A

Plasmid

#60654

Purpose

GFP-Ago2 S387A mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Mutation

S387A

Promoter

EF1a

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2-Sox9 T236A

Plasmid

#111455

Purpose

Transient expression of Flag-tagged human Sox9 with T236A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Tags

Flag

Expression

Mammalian

Mutation

T236A mutation

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGV3341

Plasmid

#170279

Purpose

Expression of mutant HIV-2 RT p66 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p66 (mutant)

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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p2R3a-3xVenusYFP-OcsT

Plasmid

#71271

Purpose

Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

3 times VenusYFP

Use

Gateway Cloning

Tags

octaline synthase terminator

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGV3340

Plasmid

#170278

Purpose

Expression of mutant HIV-2 RT p51 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p51 (mutant)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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10XUAS IVS ChrimsonR mCherry_tr

Plasmid

#111547

Purpose

Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-CDC27 wt

Plasmid

#31673

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog (CDC27 Human)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pGV3319

Plasmid

#170277

Purpose

Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

Bst-LF D720A

Tags

Hexahistidine

Expression

Bacterial

Mutation

D720A

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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FCK-Chronos-GFP

Plasmid

#80902

Purpose

Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2-Sox9 T240A

Plasmid

#111457

Purpose

Transient expression of Flag-tagged human Sox9 with T240A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Tags

Flag

Expression

Mammalian

Mutation

T240A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc-ns

Plasmid

#226719

Purpose

Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Tags

HA tag

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn1-AsCpf1(RR)

Plasmid

#109325

Purpose

AAV vector expressing AsCpf1 (RR variant)

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

hAsCpf1(RR)

Use

AAV

Tags

HA and NLS

Mutation

S542R/K607R

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HSV.S_mCherry-NLS

Plasmid

#178224

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HSV.S and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2-Sox9 T236A/T240A

Plasmid

#111458

Purpose

Transient expression of Flag-tagged human Sox9 with T236A/T240A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Tags

Flag

Expression

Mammalian

Mutation

T236A and T240A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pECE-M2-AMPKalpha2 M93G

Plasmid

#31653

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pLV EF1A mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140

Plasmid

#136601

Purpose

Lentiviral vector encoding mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140 under the EF1A promoter.

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

N-mSOG-FRB-P2A-T2A-FKBP-C-mSOG

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAR-9.47_VP2_AS

Plasmid

#74241

Purpose

Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47-VP2_AS

Use

AAV

Tags

19 alanines fused to VP2 of AAV9.47

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.NWS.Ollas_mCherry-NLS

Plasmid

#178263

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.NWS.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_NWS.HA.V5_mCherry-NLS

Plasmid

#178283

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

NWS.HA.V5 and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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