We narrowed to 5,838 results for: dna plasmids

1. pAIP4_1AKZ

   Plasmid

   #234172

   Purpose

   Heterologous protein expression of uracil-DNA glycosylase (UDG) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1AKZ (UNG Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. IpO

   Plasmid

   #48233

   Purpose

   Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

   Inserts

   URA3 3' fragment
   URA3 5' fragment
   

   Use

   Routine cloning vector

   Mutation

   -242 upstream to URA3 ORF +495 and URA3 ORF from …

   Promoter

   URA3 promoter and none

   Available Since

   Feb. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpen-RNAse A

   Plasmid

   #165565

   Purpose

   Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   RNAse A
   

   Use

   Synthetic Biology

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLSLGFP

   Plasmid

   #51494

   Purpose

   pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   GFP
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   NLS and flag

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLSLT

   Plasmid

   #51498

   Purpose

   pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   T30 TALEN pair
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9_T2A_mCherry sgAAVS1

   Plasmid

   #192480

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_T2A_iRFP670 sgAAVS1

   Plasmid

   #192477

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9_T2A_mCherry sgBFP

    Plasmid

    #192929

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-SYN-PIK3CD

    Plasmid

    #203730

    Purpose

    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid

    #89180

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLSLGUS

    Plasmid

    #51517

    Purpose

    pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    GUS
    3kb filler sequence
    

    Use

    Plant t-dna plasmid

    Tags

    NLS and flag

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLSLZ.D

    Plasmid

    #51496

    Purpose

    pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAH121

    Plasmid

    #164901

    Purpose

    The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

    Insert

    phi21 Integrase
    

    Expression

    Bacterial

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4092677

    Plasmid

    #153883

    Purpose

    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stop

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    fragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    wt;handful of SNPs as watermarks

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pSG7084 pG46-46

    Plasmid

    #232621

    Purpose

    pG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).

    Depositor

    Stephan Gruber

    Type

    Empty backbone

    Use

    Precursor plasmid to generate modified dna circles

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-sCAG-eGFP-ACAP1

    Plasmid

    #203800

    Purpose

    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    ACAP1 (ACAP1 Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-sCAG-eGFP-RAB11

    Plasmid

    #203799

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-SYN-TdTomato-RAB11

    Plasmid

    #203732

    Purpose

    AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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