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pdCas9-bacteria

Plasmid

#44249

Purpose

aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 (bacteria)

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

pLtetO-1

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Neo DEST (w531-1)

Plasmid

#19067

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase

Plasmid

#87741

Purpose

IPTG-inducible expression of T4 DNA ligase for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMIIS644

Plasmid

#238209

Purpose

Expresses native FpTasA TIGR locus. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

FpTasA

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pR26 iBFP Dest

Plasmid

#74284

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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prCAG-MS2

Plasmid

#191121

Purpose

Transcription of 47xCAG and 12xMS2 repeats from a tetracycline-inducible promoter

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Inserts

rCAG
MS2

Use

Synthetic Biology

Promoter

Tet

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHisIHFαIHFβ

Plasmid

#149384

Purpose

Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Inserts

Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta

Tags

6xHis tag and HRV3C cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCAM-kan-R4R3

Plasmid

#71275

Purpose

Binary destination vector for plant transformation. Kanamycin resistance.

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Type

Empty backbone

Use

Multisite gateway

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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