We narrowed to 8,596 results for: Set

1. pOCC254

   Plasmid

   #118889

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; mCherry, cl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS315-48nt-telo-I-SceI

   Plasmid

   #67623

   Purpose

   telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   3' gal1 unique sequence
   ura4 (ura4 Fission Yeast)
   48 bp telomere repeats
   hph
   

   Use

   Yeast cen/ars plasmid

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

   Plasmid

   #168108

   Purpose

   Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

   Plasmid

   #140204

   Purpose

   Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Step 1 of cloning c…

   Promoter

   Aspergillus fumigatus U3 (RNAPIII).

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW1759

   Plasmid

   #154315

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::TEV::AID*::3xFLAG for NT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ02B2Gm_AE

   Plasmid

   #66063

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ02B2Gm_EF

   Plasmid

   #66064

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRII-Topo U6-SpAi9L-U6SpDMDL

   Plasmid

   #78608

   Purpose

   Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SpAi9L-U6SpDMDL
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pCRII-Topo U6-SpAi9R-U6-SpDMDR

   Plasmid

   #78609

   Purpose

   Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SpAi9R-U6-SpDMDR
   

   Use

   Unspecified

   Promoter

   U6

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

    Plasmid

    #160575

    Purpose

    LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtU6-26:LbCas12a_DR
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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