We narrowed to 5,965 results for: crispr cas9 expression plasmids

1. MSP2440

   Plasmid

   #72250

   Purpose

   Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTL-Bag3-gRNA3

   Plasmid

   #196254

   Purpose

   Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTL-Bag2-gRNA2

   Plasmid

   #196252

   Purpose

   Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTL-Bag1-gRNA1

   Plasmid

   #196251

   Purpose

   Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTL-Bag4-gRNA4

   Plasmid

   #196255

   Purpose

   Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CMV-dSa VP64 Hbb

   Plasmid

   #99693

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LRG (Lenti_sgRNA_EFS_GFP)

   Plasmid

   #65656

   Purpose

   Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter-driven sgRNA expression and EFS promo…

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

   Plasmid

   #68356

   Purpose

   -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
   FlpO recombinase
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Mutation

   Promoter

   Synthetic Probasin ARRx2 and U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMTF1.1.0-gDNA

   Plasmid

   #113797

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1

   Depositor

   Kevin White

   Insert

   MTF1 (MTF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZBTB9.1.0-gDNA

    Plasmid

    #112469

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB9

    Depositor

    Kevin White

    Insert

    ZBTB9 (ZBTB9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZBTB5.1.0-gDNA

    Plasmid

    #112399

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB5

    Depositor

    Kevin White

    Insert

    ZBTB5 (ZBTB5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZNF436.1.0-gDNA

    Plasmid

    #113767

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF436

    Depositor

    Kevin White

    Insert

    ZNF436 (ZNF436 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZFX.1.0-gDNA

    Plasmid

    #112462

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZFX

    Depositor

    Kevin White

    Insert

    ZFX (ZFX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXD1.1.0-gDNA

    Plasmid

    #112446

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MXD1

    Depositor

    Kevin White

    Insert

    MXD1 (MXD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUSF2.1.0-gDNA

    Plasmid

    #112457

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor USF2

    Depositor

    Kevin White

    Insert

    USF2 (USF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZNF331.1.0-gDNA

    Plasmid

    #112461

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF331

    Depositor

    Kevin White

    Insert

    ZNF331 (ZNF331 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVEZF1.1.0-gDNA

    Plasmid

    #112437

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor VEZF1

    Depositor

    Kevin White

    Insert

    VEZF1 (VEZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZNF416.1.0-gDNA

    Plasmid

    #112468

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF416

    Depositor

    Kevin White

    Insert

    ZNF416 (ZNF416 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNFXL1.1.0-gDNA

    Plasmid

    #112451

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NFXL1

    Depositor

    Kevin White

    Insert

    NFXL1 (NFXL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOVOL1.1.0-gDNA

    Plasmid

    #113794

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor OVOL1

    Depositor

    Kevin White

    Insert

    OVOL1 (OVOL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge