We narrowed to 11,962 results for: pcDNA

1. pcDNA3-mitoExRai-AKAR2

   Plasmid

   #161757

   Purpose

   ExRai-AKAR2 targeted to the outer mitochondrial membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   DAKAP-ExRai-AKAR2
   

   Use

   Tags

   N-terminal 30 amino acids from DAKAP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-endo-GsCT

   Plasmid

   #205516

   Purpose

   Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-GsCT (GNAS Human, Synthetic)
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT/Flag-WDR5

   Plasmid

   #29759

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)-FluoSTEP-ICUE

   Plasmid

   #181845

   Purpose

   Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-ICUE (RAPGEF3 Human)
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PBD-EYFP

   Plasmid

   #13723

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   PBD-EYFP (PAK1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   p21 binding domain of human Pak1(amino acids 65-1…

   Promoter

   Available since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Flag-gp130dimer

   Plasmid

   #174394

   Purpose

   Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   IL6ST cytosolic domain with leucine zipper & C-C linker
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3puro-mGFP-ROCK2

   Plasmid

   #225472

   Purpose

   Express mEGFP-fusion protein of ROCK2

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   ROCK2 (ROCK2 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-erExRai-AKAR2

   Plasmid

   #161756

   Purpose

   ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   CYP450-ExRai-AKAR2
   

   Use

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)-FluoSTEP-AKAR

   Plasmid

   #181844

   Purpose

   Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-AKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-mito-GreenPy1High

    Plasmid

    #239070

    Purpose

    Mammalian expression of mitochondria-targeted high affinity green fluorescent pyruvate biosensor GreenPy1High

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1High
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-mito-GreenPy1Lowest

    Plasmid

    #239072

    Purpose

    Mammalian expression of mitochondria-targeted lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Lowest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-mito-GreenPy1Low

    Plasmid

    #239071

    Purpose

    Mammalian expression of mitochondria-targeted low affinity green fluorescent pyruvate biosensor GreenPy1Low

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Low
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-mito-dGreenPy1

    Plasmid

    #239073

    Purpose

    Mammalian expression of mitochondria-targeted green fluorescent pyruvate control biosensor dGreenPy1

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    dGreenPy1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO GABARAP

    Plasmid

    #218427

    Purpose

    Expression of tagless GABARAP

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-Ikkb-FLAG WT

    Plasmid

    #23298

    Depositor

    Warner Greene

    Article

    Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

    Insert

    Ikkbeta (IKBKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3/Myc-DNMT3B1

    Plasmid

    #35522

    Purpose

    Mammalian expression of human DNMT3B1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B1 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-N-Flag-NLRP3

    Plasmid

    #75127

    Purpose

    Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1_GEM2-Halo-FRB

    Plasmid

    #197056

    Purpose

    Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 FLAG FBXW7alpha

    Plasmid

    #236470

    Purpose

    transient overexpression of FBXW7alpha in mammalian cells

    Depositor

    Michele Pagano

    Article

    Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

    Insert

    FBXW7alpha (FBXW7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-TfR-OVA

    Plasmid

    #64600

    Purpose

    Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Transmembrane domain of Transferrin Receptor (TfR…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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