165,528 results

1. hfCas12Max

   Plasmid

   #195336

   Purpose

   vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized hfCas12Max
   

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   N243R, E336R, D892R

   Promoter

   CBh, CMV, hU6

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

   Plasmid

   #216156

   Purpose

   Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with C-terminal FLAG tag
   

   Expression

   Mammalian

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO GFP11-LRRK2_G2019S

   Plasmid

   #231175

   Purpose

   Expresses GFP-11-LRRK2 G2019S in mammalian cells

   Depositor

   Samara Reck-Peterson

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   GFP11-LRRK2 (G2019S) (LRRK2 Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   G2019S

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AiP12689 - pAAV-AiE0682h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2689)

   Plasmid

   #191727

   Purpose

   AI plasmid ALIAS: AiP12689. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   none

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. COC053

   Plasmid

   #209131

   Purpose

   Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

   Depositor

   Christopher A. O'Callaghan

   Article

   O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

   Insert

   Enzyme BirA (birA E. coli)
   

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+mScarlet3-C Cloning Vector

   Plasmid

   #220616

   Purpose

   To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   mScarlet3 fusion

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-IRF9

   Plasmid

   #71452

   Purpose

   Lentiviral expression of IRF9

   Depositor

   George Stark

   Article

   Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

   Insert

   IRF9 (IRF9 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-FLAG-S6K1

   Plasmid

   #100509

   Purpose

   Expresses FLAG tagged S6K1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-BP1-2 pLPC-Puro

    Plasmid

    #19835

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

    Insert

    Fragment of p53-Binding Protein 1 (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Mutation

    Fragment of human 53BP1 aa 1220 - 1711

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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