We narrowed to 8,596 results for: Set

1. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pv9_CBX1_2xChromo-13XL-BASU

   Plasmid

   #179412

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1.2xChromo-13XL-BASU
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. iCas

   Plasmid

   #84232

   Purpose

   Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

   Depositor

   Meng How Tan

   Article

   Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

   Insert

   Cas9
   

   Tags

   2A-OFP co-expression and ERT2-ERT2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL3.7

   Plasmid

   #11795

   Purpose

   3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

   Depositor

   Luk Parijs

   Article

   Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. HR180-LGR5-GFP

   Plasmid

   #129092

   Purpose

   KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

   Depositor

   Toshiro Sato

   Article

   Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

   Insert

   EGFP and LGR5 homology arms (LGR5 Human)
   

   Use

   Homologous recombination donor plasmid

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGRG36-Kn-PA1-GFP

   Plasmid

   #79088

   Purpose

   pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

   Depositor

   Frank Rosenzweig

   Article

   Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

   Insert

   GFP
   

   Use

   Bacterial chromosome integration and expression o…

   Expression

   Bacterial

   Promoter

   PA1

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

   Plasmid

   #201684

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1 (V106W)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR-mcBEST

   Plasmid

   #209416

   Purpose

   Plasmid for multiplexed cytosine base editing in streptomycetes

   Depositor

   Tilmann Weber

   Article

   Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

   Inserts

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   csy4 from Pseudomonas aeruginosa PAO1
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   base editing cassette: PtipA ; sgRNAs: PkasO* an…

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AID-mTagBFP2-loxP_myo2_neoR-loxP

   Plasmid

   #194055

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Tags

   codon optimized mTagBFP2

   Expression

   Worm

   Promoter

   None

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184741

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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