162,981 results

1. pCGN-MEF2B

   Plasmid

   #32959

   Purpose

   For mammalian expression of human MEF2B with an HA tag

   Depositor

   Ron Prywes

   Article

   Han et al Mol Cell Biol. 1995 Jun;15(6):2907-15.

   Insert

   MEF2B (MEF2B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23-GW

   Plasmid

   #60323

   Purpose

   This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmRFP-LC3

   Plasmid

   #21075

   Purpose

   Mammalian expression of rat LC3 fused to mRFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS

   Plasmid

   #238243

   Purpose

   For integration of mCherry_P2AT2A_GFP-nb-2xKS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Casp3 C163A-myc

   Plasmid

   #11814

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

   Insert

   Caspase 3 catalytic mutant (CASP3 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C163A

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-AXL-V5/HIS

   Plasmid

   #200117

   Purpose

   Expression of human AXL receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human AXL receptor tyrosine kinase (AXL Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC28-MacroGreen

   Plasmid

   #160665

   Purpose

   Expresses GFP fusion of a multisite mutant of the Af1521 protein

   Depositor

   Herwig Schüler

   Article

   Garcia-Saura et al Commun Biol. 2021 Jul 28;4(1):919. doi:

   Insert

   Af1521 modified synthetic construct eGFP fusion
   

   Use

   Tags

   eGFP and hexahistidine_TEV recognition

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-ABCC1_STOP

   Plasmid

   #221423

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

   Insert

   ABCC1 (ABCC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

   Plasmid

   #187336

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTECH-chPylRS(IPYE)

    Plasmid

    #99222

    Purpose

    Expression plasmid encoding chPylRS(IPYE) and pylT

    Depositor

    Dieter Söll

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chPyl(IPYE)RS; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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