We narrowed to 2,684 results for: Kl
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Plasmid#98098PurposeaBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio aBb-crystallin 1 kb fragment (-1074/-1)Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on-puromycin-shTRX1-259
Plasmid#110920PurposeTo knockdown Thioredoxin-1 following doxycycline additionDepositorInsertTRX1 (TXN Human)
UseLentiviralTagsExpressionMutationPromoterunknownAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS663-pMCSG7-AcrIIC4Hpa
Plasmid#113440PurposeExpresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cellsDepositorInsertType II-C anti-CRISPR with N-terminal 6xHis and TEV site
UseTags6xHisExpressionBacterialMutationPromoterlacIAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS664-pMCSG7-AcrIIC5Smu
Plasmid#113441PurposeExpresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cellsDepositorInsertType II-C anti-CRISPR with N-terminal 6xHis and TEV site
UseTags6xHisExpressionBacterialMutationPromoterlacIAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
AIDx-ID-1058
Plasmid#135347PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminusDepositorInsertnSpyCas9-hAIDx-ID-1058-UGI
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEMAX-NLS-P2A_BLAST
Plasmid#135355PurposeExpression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertABEMax-2XNLS
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(dAB)-luc
Plasmid#12423DepositorInsertM-CSF receptor promoter fragment (CSF1 Human)
UseLuciferaseTagsExpressionMutationNo AML1 and C/EBP binding sites (delete bp -86 to…PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Ba-crystallin 3kb promoter/AcGFP
Plasmid#98097PurposeaBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Ba-crystallin 3 kb fragment (-3000/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS
Plasmid#113437PurposeExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgGCAT_2
Plasmid#72885PurposeCRISPR-Cas9 induced gene disruptionDepositorInsertsgGCAT
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCSG49-AlleyCat7
Plasmid#42591DepositorInsertmutated C-terminal portion of calmodulin gene (M76-K148)
UseTagsHis and TEVExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Isole…PromoterT7Available sinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIA123
Plasmid#234038PurposeE. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.DepositorInsertsCas9
sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPgdh and PxylAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MfnG-EcYRS-EGFP*
Plasmid#191119PurposeA plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafishDepositorInsertMfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DXO
Plasmid#177993PurposeFor purification of N-terminally His-tagged DXO from bacteriaDepositorInsertDXO (DXO Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Control-Control-LRG-GFP
Plasmid#225873PurposeTwo copies of guide RNA targeting a control sequenceDepositorInsertControl
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2
Plasmid#215699PurposeProduces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1DepositorInsertCHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 SS/R
Plasmid#166976PurposeExpresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 AS/Y
Plasmid#166974PurposeExpresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 AQ/Y
Plasmid#166975PurposeExpresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 AIDx no UGI
Plasmid#135349PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.DepositorInsertnSpyCas9-hAIDx-ID-1058
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only