We narrowed to 34,434 results for: CaS

1. pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD

   Plasmid

   #200845

   Purpose

   Inducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSIP124: pDonor(CyCAST)_0.5kb-cargo

   Plasmid

   #200848

   Purpose

   CyCAST donor harboring non-functional cargo.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSIP122: pHelper(CyCAST)_Shuffled-spacer

   Plasmid

   #200841

   Purpose

   Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   CyCAST
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-EFS-DjCas13d-P2A-mCherry-pA

   Plasmid

   #233027

   Purpose

   To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   DjCas13d/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-EFS-CasRx-P2A-mCherry-pA

   Plasmid

   #233025

   Purpose

   To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   CasRx/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-EFS-hfCas13d-P2A-mCherry-pA

   Plasmid

   #233026

   Purpose

   To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   hfCas13d/mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 Cas9-P2A-dTomato-T20

   Plasmid

   #231912

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-CasRx-non-targeting (pEZ18)

   Plasmid

   #229049

   Purpose

   Cloning backbone for CasRx crRNA (U6 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-Cas13bt1-non-targeting (pJK2)

    Plasmid

    #229054

    Purpose

    Cloning backbone for Cas13bt1 crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. phU1-Cas13bt1-non-targeting (pEZ25)

    Plasmid

    #229055

    Purpose

    Cloning backbone for Cas13bt1 crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-RanCas13b-non-targeting (pJK6)

    Plasmid

    #229056

    Purpose

    Cloning backbone for RanCas13b crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. phU1-RanCas13b-non-targeting (pEZ45)

    Plasmid

    #229057

    Purpose

    Cloning backbone for RanCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid

    #221537

    Purpose

    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid

    #221540

    Purpose

    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 1203AB-CMV-Cas7.1-H43A-mutant

    Plasmid

    #221541

    Purpose

    Effector expresses Cas7.1 (truncated from DiCas7-11), H to A mutation at amino acid 43

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1203AC-CMV-Cas7.1-Y55A-mutant

    Plasmid

    #221542

    Purpose

    Effector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1203AD-CMV-Cas7.1-N152A-mutant

    Plasmid

    #221543

    Purpose

    ffector expresses Cas7.1 domain (truncated from DiCas7-11), N to A mutation at amino acid 152

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1203O-CMV-DiCas7-11-Δ7.1-bGHpolyA

    Plasmid

    #220959

    Purpose

    DiCas7-11 effector with Cas7.1 domain deletion mutation

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1203W-CMV-Cas7.1-7.2-bGHpolyA

    Plasmid

    #220961

    Purpose

    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLN34 - pBait-5GC[1xMS2]-McaS

    Plasmid

    #222403

    Purpose

    arabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

    Depositor

    Katherine Berry

    Article

    Linh D. Nguyen et al Sci Rep 15, 3820 (2025)

    Insert

    McaS (mcaS E. coli)
    

    Use

    Tags

    1xMS2 hairpin, 5GC clamp

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC130: pAAV.CMV-CasRx-VEGFA sgRNA

    Plasmid

    #203443

    Purpose

    Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA
    RfxCas13d
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

    Plasmid

    #203447

    Purpose

    Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

    Plasmid

    #203451

    Purpose

    Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-non targeting (NT) sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimised using GenScript tool

    Promoter

    CMV and U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

    Plasmid

    #227800

    Purpose

    Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA 2 (Vegfa Synthetic)
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

    Plasmid

    #227801

    Purpose

    Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA 3 (Vegfa Synthetic)
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

    Plasmid

    #222107

    Purpose

    Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

    Depositor

    Jonathan Weissman

    Article

    She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

    Insert

    CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
    

    Use

    AAV and CRISPR

    Tags

    HA and P2A-mCherry

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAVS1_dCas9-XTEN-KRAB-p2A-GFP

    Plasmid

    #222108

    Purpose

    Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

    Depositor

    Jonathan Weissman

    Article

    She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

    Insert

    CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
    

    Use

    AAV and CRISPR

    Tags

    HA and P2A-GFP

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBK1302-AAV-EFSNC-dCjCas9-KRAB

    Plasmid

    #223147

    Purpose

    Expression of KRAB with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBK1312-AAV-EFSNC-dSaCas9-KRAB

    Plasmid

    #223157

    Purpose

    Expression of KRAB with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Anti-CASPR/Neurexin IV [K65A/30R]

    Plasmid

    #219448

    Purpose

    Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat) IgG2a R-mAb. Derived from hybridoma K65A/30.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Anti-CASPR/Neurexin IV [K65A/43R]

    Plasmid

    #219449

    Purpose

    Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat) IgG2a R-mAb. Derived from hybridoma K65A/43.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

    Plasmid

    #220494

    Purpose

    To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting LIG4 exon 3 (LIG4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJS-BCD-Strep-PCaspase-R153A

    Plasmid

    #214036

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R153A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMSCV-Myc-Caspase-11_humanized-mutant

    Plasmid

    #214316

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (Casp4 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    KLS288YKT_KASIHS348TPRAKA

    Promoter

    MESV

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

    Plasmid

    #209197

    Purpose

    Mutagenesis of Faah

    Depositor

    Larry Zweifel

    Article

    Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

    Insert

    Faah (Faah Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

    Plasmid

    #209199

    Purpose

    Mutagenesis of Kcnma1

    Depositor

    Larry Zweifel

    Article

    Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

    Insert

    Kcnma1 (Kcnma1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Anti-CASPR/Neurexin IV [K65/35R-1]

    Plasmid

    #206683

    Purpose

    Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35-1.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant Mouse monoclonal antibody (Cntnap1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. A3Bctd-L7G-Cas9n-UGI-NLS

    Plasmid

    #198886

    Purpose

    Express A3Bctd-L7G BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Loop 7 of A3G

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. A3Bctd-D314E-Cas9n-UGI-NLS

    Plasmid

    #198887

    Purpose

    Express A3Bctd-D314E BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D314E

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PET-B-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207388

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A, amino acids 10…

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. PET-B-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207378

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. PET-Blackjack SpCas9-NLS-6xHis

    Plasmid

    #207375

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Blackjack SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Anti-CASPR/Neurexin IV [K65/35R-2b]

    Plasmid

    #199403

    Purpose

    Mammalian expression plasmid of Anti-CASPR/Neurexin IV [K65/35R-2b] (Rat). Derived from hybridoma K65/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-CASPR/Neurexin IV (Rat) recombinant mouse monoclonal antibody (Cntnap1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge