We narrowed to 5,495 results for: IRES

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pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

Plasmid

#193298

Purpose

episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

CAG

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCSC-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90214

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-Empty Vector-IRES-Puro

Plasmid

#120568

Purpose

For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Type

Empty backbone

Use

Lentiviral

Tags

Flag-HA-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

Plasmid

#209410

Purpose

Expression of wild-type neonatal isoform of human SCN2A.

Depositor

Al George

Article

Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

Insert

SCN2A Variant 4, stabilized (SCN2A Human)

Expression

Mammalian

Mutation

IVS intron inserted after bp 4551 of the SCN2A OR…

Promoter

CMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJRH-1364 LV EF1a-CD4 IRES-EGFP

Plasmid

#201920

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD4 (CD4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

Plasmid

#134864

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

emerin (EMD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

Plasmid

#128387

Purpose

Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption

Depositor

Craig Duvall

Article

Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

Inserts

NLuc398-3xGGGGS-LGALS8 (LGALS8 Human, Synthetic)
CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

CLuc394 and NLuc398

Expression

Mammalian

Promoter

EFS and IRES

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry-weak NLS

Plasmid

#129717

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLS

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry-weak NLS

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX

Plasmid

#208693

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3h

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

Plasmid

#122991

Purpose

Expresses Flag-tagged hRPGRIP1L in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

RPGRIP1L (RPGRIP1L Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1 alpha

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

Plasmid

#134859

Purpose

Expresses EGFP-tagged ERGIC marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

ERGIC53 (Lman1 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Gluc-RMA-IRES-EGFP

Plasmid

#189629

Purpose

Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

Gaussia luciferase fused to Fc
IRES-EGFP

Use

AAV and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Promoter

IRES and hSyn

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

Plasmid

#134865

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Membrin (GOSR2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-MitoRlucN-T2A-RlucCER-IRES-mCherry

Plasmid

#249563

Purpose

For detection of ER-mitochondrial contact

Depositor

Ginam Cho, Jeffrey Golden, Youngshin Lim

Article

Chen et al J Vis Exp. 2024 Oct 11;(212):10.3791/668

Insert

MitoRlucN-T2A-RlucCER

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Promoter

Chicken beta-actin promoter with CMV enhancer

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

Plasmid

#102886

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52-FLAG

Plasmid

#204523

Purpose

Retroviral expression of human CALR del52-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR del52-FLAG (CALR Human)

Use

Retroviral

Mutation

del52

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1a MICA*009-IRES-ZsGreen

Plasmid

#114007

Purpose

Induces expression of human MICA allele 009 cDNA

Depositor

Kai Wucherpfennig

Article

Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

Insert

MICA*009 (MICA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro HEXIM1

Plasmid

#200726

Purpose

Lentiviral expression of Flag-Tagged human HEXIM1

Depositor

Claudio Alarcón

Insert

HEXIM1 (HEXIM1 Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mCherry-MinD-IRES-Puro

Plasmid

#213112

Purpose

Expression of mCherry-MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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