We narrowed to 87,289 results for: lias

1. pAcBac3-EcTrp-TGA-EGFP**

   Plasmid

   #228786

   Purpose

   EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   EGFP**
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM 1995

   Plasmid

   #217826

   Purpose

   Expresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.

   Depositor

   Aziz Sancar

   Article

   Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9.

   Insert

   HY4 (CRY1 Mustard Weed)
   

   Use

   Tags

   Maltose-binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIR218-2_nt5G>U

   Plasmid

   #174009

   Purpose

   miR218 nt5 point mutation in SLIT3

   Depositor

   Andrew M. Siedlecki

   Article

   Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

   Insert

   SLIT3 (SLIT3, MIR218-2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NT5 G>U point mutation in SLIT3

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDN-D2irTN6kwh

   Plasmid

   #44724

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   htetR::NLS
   

   Use

   Synthetic Biology; Expression regulator/reporter;…

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-CIB1-mCherry-Rab8A

   Plasmid

   #194333

   Purpose

   For use in light-induced Rab8a protein inactivation through the interaction with CRY2

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Inserts

   CIB1 (CIB1 Mustard Weed)
   Rab8a (RAB8A Canis Lupus)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE3328

   Plasmid

   #107539

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K607R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE3329

   Plasmid

   #107538

   Purpose

   Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Lb crRNA
   hLbCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K595R

   Promoter

   CMV and human U6

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE3327

   Plasmid

   #107535

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RVR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K548V, N552R

   Promoter

   CMV and human U6

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE3334

   Plasmid

   #107537

   Purpose

   Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   hMbCpf1(RVR mutant)
   

   Use

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K582V, N586R

   Promoter

   CMV and human U6

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE3330

    Plasmid

    #107534

    Purpose

    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    hLbCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE3336

    Plasmid

    #107541

    Purpose

    Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(RR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K637R

    Promoter

    CMV and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE3333

    Plasmid

    #107540

    Purpose

    Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    hFnCpf1(RR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K671R

    Promoter

    CMV and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE3331

    Plasmid

    #107536

    Purpose

    Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    hFnCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K613V, N617R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-SAM-Puro

    Plasmid

    #198713

    Purpose

    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-SAM-Cre

    Plasmid

    #198714

    Purpose

    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pgk and U6

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pITGA5_1745G>U

    Plasmid

    #173812

    Purpose

    mir218 binding site2 point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    NT1745 ITGA5 point mutation (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    AmpR

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pITGA5_473G>A

    Plasmid

    #173811

    Purpose

    mir218 binding site1 point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    NT473 ITGA5 point mutation (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    AmpR

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pITGA5_Q853L

    Plasmid

    #174008

    Purpose

    Fibronectin receptor binding site point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    FibronR ITGA5 point mutation (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    AmpR

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CIRTS-FTO-control

    Plasmid

    #216859

    Purpose

    CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-FTO-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CIRTS-YTHDF2-control

    Plasmid

    #216857

    Purpose

    CIRTS RNA targeting system for targeted knockdown of m6A-containing transcript at the synapse. CIRTS-YTHDF2-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-YTHDF2-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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