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pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

Plasmid

#213119

Purpose

Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

Depositor

Scott Coyle

Publication

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKEW336-MBP-TEV-MbCas12a-33362

Plasmid

#115670

Purpose

Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pScaf-5544.1

Plasmid

#111405

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GH'

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SAMTOR (G172A)

Plasmid

#100514

Purpose

Expresses HA tagged SAMTOR G172A mutant in mammalian cells

Depositor

David Sabatini

Publication

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SAMTOR (BMT2 Human)

Tags

HA

Expression

Mammalian

Mutation

G172A

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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KG371-GFP-mios

Plasmid

#87052

Purpose

mammalian expression of mios

Depositor

David Sabatini

Publication

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

mios (MIOS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-ITFG2

Plasmid

#87049

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Publication

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC6-Flag CASTOR1

Plasmid

#84496

Purpose

Lentiviral expression of CASTOR1

Depositor

David Sabatini

Publication

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

sestrin2

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-Flag CASTOR1 I280A

Plasmid

#84495

Purpose

overexpression of CASTOR1 I280A mutant

Depositor

David Sabatini

Publication

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CASTOR1 I280A (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I280A

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRK5 CASTOR2-HA Cterm

Plasmid

#84489

Purpose

overexpression of CASTOR2 with HA tag

Depositor

David Sabatini

Publication

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CASTOR2 (GATSL2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pALPS vif

Plasmid

#101328

Purpose

Encodes codon optimized NL4-3 vif

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 vif

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS vpr

Plasmid

#101329

Purpose

Encodes codon optimized NL4-3 vpr

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 vpr

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3xFlag-Dam

Plasmid

#121909

Purpose

Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

Depositor

Douglas Feldman

Publication

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Dam human codon optimized (dam E. coli)

Tags

Flag

Expression

Mammalian

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.1853Gmut

Plasmid

#190479

Purpose

minigene assay

Depositor

Alfredo Brusco

Publication

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Mutation

c.1853A>G

Promoter

SV40

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAC5.1-dCry-V5His

Plasmid

#136237

Purpose

Protein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tags

Depositor

Steven Reppert, Aziz Sancar

Publication

Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

Insert

DmCry

Tags

His and V5

Expression

Insect

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-GEMSgRNA2

Plasmid

#204768

Purpose

pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

Depositor

Kate Meyer

Publication

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

guide RNA targeting GEMS m6A sensor mRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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VRER-ABEmax

Plasmid

#119812

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRER)

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_zeo_sgTP53

Plasmid

#183180

Purpose

pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Publication

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-mGFP

Plasmid

#174609

Purpose

expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

Depositor

Stanley Riddell

Publication

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD19 extracellular and transmembrane domain (Cd19 Mouse)

Tags

palmitoylated EGFP

Expression

Mammalian

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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