161,152 results

1. pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

   Plasmid

   #187576

   Purpose

   Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   LMNA (LMNA Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1105-Desmoglein2-FLAG-eGFP

   Plasmid

   #36989

   Depositor

   Kathleen Green

   Article

   Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Ep

   Insert

   Dsg2 (DSG2 Human)
   

   Use

   Tags

   FLAG and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Rab11a-7

   Plasmid

   #55124

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab11a (RAB11A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. mPlum-pBAD

   Plasmid

   #54564

   Purpose

   Localization: Protein Expression Vector , Excitation: 590, Emission: 649

   Depositor

   Michael Davidson, Roger Tsien

   Article

   Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

   Type

   Empty backbone

   Use

   Tags

   6xHis and mPlum

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CMV-eGFP

   Plasmid

   #193022

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3 Enhancer Vector

   Plasmid

   #212938

   Purpose

   Firefly luciferase vector for investigating regions controlling transcription

   Depositor

   Debrya Groskreutz

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEFBOS_CreIRESpuro

   Plasmid

   #183812

   Purpose

   Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

   Depositor

   Ed Stanley

   Article

   Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-Blasticidin

   Plasmid

   #75085

   Purpose

   retrovirus-mediated gene transfer into mammalian cells

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDY0973-RADARS iCaspase

    Plasmid

    #182539

    Purpose

    Express RADARS iCaspase that conditionally turn on iCaspase in the presence of target RNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Insert

    iCaspase9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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